RGD Reference Report - The MicroRNA-210/Casp8ap2 Pathway Alleviates Hypoxia-Induced Injury in Myocardial Cells by Regulating Apoptosis and Autophagy. - Rat Genome Database

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The MicroRNA-210/Casp8ap2 Pathway Alleviates Hypoxia-Induced Injury in Myocardial Cells by Regulating Apoptosis and Autophagy.

Authors: Li, Kunsheng  Pan, Jun  Li, Qiuchang  Li, Shiliang  Li, Kai  Cheng, Yongqing  Chai, Lin  Li, Chao  Li, Junling  Fu, Zhikun  Wang, Dongjin  Bai, Yang 
Citation: Li K, etal., Heart Surg Forum. 2020 Oct 21;23(6):E797-E802. doi: 10.1532/hsf.3173.
RGD ID: 155260322
Pubmed: PMID:33234216   (View Abstract at PubMed)
DOI: DOI:10.1532/hsf.3173   (Journal Full-text)


AIM: This study was conducted to investigate the role of the miR-210/Caspase8ap2 pathway in apoptosis and autophagy in hypoxic myocardial cells.
METHODS: The miR-control, miR-210 mimic, and miR-210 inhibitor were transfected into rat myocardial H9C2 cells. The transfection efficiency of exogenous miR-210 was determined by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). H9C2 cells were then treated with CoCl2 for 24, 48, and 72 h to generate a myocardial injury model. The apoptosis of H9C2 cells was assessed by flow cytometry. Additionally, a western blot assay was used to determine the expression of the autophagy-associated proteins light chain 3 (LC3), p62 and Beclin-1, and apoptosis-associated proteins Caspase8ap2, cleaved caspase 8, and cleaved caspase 3.
RESULTS: We determined that a 48 h hypoxia treatment duration in H9C2 cardiomyocytes induced myocardial injury. Additionally, the overexpression of miR-210 significantly inhibited cell apoptosis. MiR-210 suppressed autophagy by upregulating p62 and downregulating LC3II/I in hypoxic H9C2 cells. Caspase8ap2 was a putative target of miR-210, miR-210 mediated apoptosis, and autophagy of H9C2 cells via suppressing Caspase8ap2. Furthermore, the expression of caspase 8, caspase 3, and Beclin-1 were decreased in response to miR-210.
CONCLUSION: miR-210 exhibits anti-apoptosis and anti-autophagy effects, which alleviate myocardial injury in response to hypoxia.

Gene-Chemical Interaction Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cobalt dichloride increases expression ISOMir210 (Rattus norvegicus)155260322; 155260322cobalt dichloride increases expression of Mir210 miRNA in cardiomyocytesRGD 
cobalt dichloride increases expression EXP 155260322cobalt dichloride increases expression of Mir210 miRNA in cardiomyocytesRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to hypoxia  IEP 155260322 RGD 
regulation of cardiac muscle cell apoptotic process  IMP 155260322 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mir210  (microRNA 210)

Genes (Mus musculus)
Mir210  (microRNA 210)

Genes (Homo sapiens)
MIR210  (microRNA 210)


Additional Information