RGD Reference Report - Overexpression of MicroRNA-145 Promotes Ascending Aortic Aneurysm Media Remodeling through TGF-β1. - Rat Genome Database

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Overexpression of MicroRNA-145 Promotes Ascending Aortic Aneurysm Media Remodeling through TGF-β1.

Authors: Pei, H  Tian, C  Sun, X  Qian, X  Liu, P  Liu, W  Chang, Q 
Citation: Pei H, etal., Eur J Vasc Endovasc Surg. 2015 Jan;49(1):52-9. doi: 10.1016/j.ejvs.2014.10.018.
RGD ID: 155260284
Pubmed: PMID:25465469   (View Abstract at PubMed)
DOI: DOI:10.1016/j.ejvs.2014.10.018   (Journal Full-text)


OBJECTIVE: In this study the role of microRNA-145 expression in ascending aortic aneurysm wall media remodeling was examined.
METHODS: Aortic wall samples were obtained from 10 patients who had undergone surgery for ascending aortic aneurysm. Control aortic tissue samples were obtained from 10 patients who had undergone coronary artery bypass graft. The levels of microRNA-145 were analyzed using real time quantitative reverse transcriptase polymerase chain reaction, and western blots were used to determine the expressions of osteopontin (OPN) and collagen. In vitro cultures of vascular smooth muscle cells (VSMCs) were established from both patient groups. The VSMCs were transfected with either microRNA-145 mimics or microRNA-145 inhibitors to determine the effect of microRNA-145 on the expression of OPN and collagen. Furthermore, cells were co-transfected with microRNA-145 mimics and TGF-b1 siRNA to investigate whether TGF-b1 is involved in the process that microRNA-145 increases the expression of OPN and collagen.
RESULTS: Aortic microRNA-145, OPN and collagen III was increased in ascending aortic aneurysm patients compared with controls (p < .05). VSMCs transfected with microRNA-145 mimics increased the expression of both OPN (average of 1.59-fold, p < .05) and collagen III (mean of 1.71-fold, p < .05). Cells transfected with microRNA-145 inhibitors decreased expression of both OPN and collagen III compared with negative controls. In addition, the inhibition of TGF-b1 decreased the positive effect of microRNA-145 on the expression of OPN and collagen III.
CONCLUSION: The increased expression of microRNA-145 promotes media remodeling through TGF-b1 in the aortic aneurysm wall.



RGD Manual Disease Annotations    Click to see Annotation Detail View
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MIR145Humanaortic aneurysm disease_progressionIEP miRNA:increased expression:aorta (human)RGD 
Mir145Rataortic aneurysm disease_progressionISOMIR145 (Homo sapiens)miRNA:increased expression:aorta (human)RGD 
Mir145aMouseaortic aneurysm disease_progressionISOMIR145 (Homo sapiens)miRNA:increased expression:aorta (human)RGD 
SPP1Humanaortic aneurysm  IEP protein:increased expression:aorta (human)RGD 
Spp1Rataortic aneurysm  ISOSPP1 (Homo sapiens)protein:increased expression:aorta (human)RGD 
Spp1Mouseaortic aneurysm  ISOSPP1 (Homo sapiens)protein:increased expression:aorta (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mir145  (microRNA 145)
Spp1  (secreted phosphoprotein 1)

Genes (Mus musculus)
Mir145a  (microRNA 145a)
Spp1  (secreted phosphoprotein 1)

Genes (Homo sapiens)
MIR145  (microRNA 145)
SPP1  (secreted phosphoprotein 1)


Additional Information