RGD Reference Report - Integrating DNA methylation and microRNA biomarkers in sputum for lung cancer detection. - Rat Genome Database

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Integrating DNA methylation and microRNA biomarkers in sputum for lung cancer detection.

Authors: Su, Yun  Fang, HongBin  Jiang, Feng 
Citation: Su Y, etal., Clin Epigenetics. 2016 Oct 19;8:109. doi: 10.1186/s13148-016-0275-5. eCollection 2016.
RGD ID: 152998954
Pubmed: PMID:27777637   (View Abstract at PubMed)
PMCID: PMC5070138   (View Article at PubMed Central)
DOI: DOI:10.1186/s13148-016-0275-5   (Journal Full-text)


BACKGROUND: Abnormal microRNA (miRNA) expressions and promoter methylation of genes detected in sputum may provide biomarkers for non-small lung cancer (NSCLC). Here, we evaluate the individual and combined analysis of the two classes of sputum molecular biomarkers for NSCLC detection.
RESULTS: We analyze expression of 3 miRNAs (miR-21, miR-31, and miR-210) and methylation of 3 genes (RASSF1A, PRDM14, and 3OST2), which were previously identified as potential biomarkers for NSCLC, in sputum of a set of 117 stage I NSCLC patients and 174 cancer-free smokers. The results are validated in a different set of 144 stage I NSCLC patients and 171 controls. The panel of 3 miRNA biomarkers has 81.5 % sensitivity and 85.9 % specificity; the panel of 3 methylation biomarkers displays 82.9 % sensitivity and 76.4 % specificity for NSCLC detection. Integrated analysis of 2 miRNAs (miR-31 and miR-210) and 2 genes (RASSF1A and 3OST2) yields higher sensitivity (87.3 %) and specificity (90.3 %) compared with the individual panels of the biomarkers (P < 0.05). Combined analysis of all the 3 miRNAs and 3 genes does not have performance superior to that of the panel of 2 miRNAs and 2 genes (P > 0.05). The performance of combined use of the two classes of biomarkers was confirmed in the validation set.
CONCLUSIONS: The integration of two different classes of biomarkers synergistically improves both the sensitivity and the specificity for the early detection of NSCLC.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
lung adenocarcinoma  ISOMir21a (Mus musculus)152998954; 152998954miRNA:increased expression:sputum (human)RGD 
lung adenocarcinoma  IEP 152998954miRNA:increased expression:sputum (human)RGD 
lung non-small cell carcinoma  IDA 152998954; 152998954; 152998954DNA:hypermethylationRGD 
lung non-small cell carcinoma  ISOHS3ST2 (Homo sapiens)152998954; 152998954DNA:hypermethylationRGD 
lung non-small cell carcinoma  IEP 152998954miRNA:increased expression:sputum (human)RGD 
lung non-small cell carcinoma  ISOMIR31 (Homo sapiens)152998954; 152998954miRNA:increased expression:sputum (human)RGD 
lung non-small cell carcinoma  ISOPRDM14 (Homo sapiens)152998954; 152998954DNA:hypermethylationRGD 
lung non-small cell carcinoma  ISORASSF1 (Homo sapiens)152998954DNA:hypermethylationRGD 
lung squamous cell carcinoma  IEP 152998954miRNA:increased expression:sputum (human)RGD 
lung squamous cell carcinoma  ISOMIR210 (Homo sapiens)152998954; 152998954miRNA:increased expression:sputum (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Hs3st2  (heparan sulfate-glucosamine 3-sulfotransferase 2)
Mir21  (microRNA 21)
Mir210  (microRNA 210)
Mir31  (microRNA 31)
Prdm14  (PR/SET domain 14)

Genes (Mus musculus)
Hs3st2  (heparan sulfate (glucosamine) 3-O-sulfotransferase 2)
Mir210  (microRNA 210)
Mir21a  (microRNA 21a)
Mir31  (microRNA 31)
Prdm14  (PR domain containing 14)
Rassf1  (Ras association (RalGDS/AF-6) domain family member 1)

Genes (Homo sapiens)
HS3ST2  (heparan sulfate-glucosamine 3-sulfotransferase 2)
MIR21  (microRNA 21)
MIR210  (microRNA 210)
MIR31  (microRNA 31)
PRDM14  (PR/SET domain 14)
RASSF1  (Ras association domain family member 1)


Additional Information