RGD Reference Report - Diagnostic potential of miR-126, miR-143, miR-145, and miR-652 in malignant pleural mesothelioma. - Rat Genome Database

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Diagnostic potential of miR-126, miR-143, miR-145, and miR-652 in malignant pleural mesothelioma.

Authors: Andersen, Morten  Grauslund, Morten  Ravn, Jesper  Sørensen, Jens B  Andersen, Claus B  Santoni-Rugiu, Eric 
Citation: Andersen M, etal., J Mol Diagn. 2014 Jul;16(4):418-30. doi: 10.1016/j.jmoldx.2014.03.002. Epub 2014 Jun 6.
RGD ID: 151660330
Pubmed: PMID:24912849   (View Abstract at PubMed)
DOI: DOI:10.1016/j.jmoldx.2014.03.002   (Journal Full-text)

Malignant pleural mesothelioma (MPM) is difficult to distinguish from reactive mesothelial proliferations (RMPs). It is uncertain whether miRNAs are useful biomarkers for differentiating MPM from RMPs. Thus, we screened with a quantitative RT-PCR (RT-qPCR)-based platform the expression of 742 miRNAs in formalin-fixed, paraffin-embedded, preoperative diagnostic biopsy samples, surgically resected MPM specimens previously treated with chemotherapy, and corresponding non-neoplastic pleura (NNP), from five patients. miR-126, miR-143, miR-145, and miR-652 were significantly down-regulated (>=twofold) in resected MPM and/or chemotherapy-naïve diagnostic tumor biopsy samples. The miRNA expression pattern was validated by RT-qPCR in a cohort of 40 independent MPMs. By performing binary logistic regression on the RT-qPCR data for the four miRNAs, the established four-miRNA classifier differentiated MPM from NNP with high sensitivity and specificity (area under the curve, 0.96; 95% CI, 0.92-1.00). The classifier's optimal logit(P) value of 0.62 separated NNP and MPM samples with a sensitivity of 0.95 (95% CI, 0.89-1.00), a specificity of 0.93 (95% CI, 0.87-0.99), and an overall accuracy of 0.94 (95% CI, 0.88-1.00). The level of miR-126 in MPM was inversely correlated with that of the known target, the large neutral amino acid transporter, small subunit 1 (r = -0.38; 95% CI, -0.63 to -0.06). Overall, these results indicate that these four miRNAs may be suitable biomarkers for distinguishing MPM from RMPs.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
malignant pleural mesothelioma  IEP 151660330protein:increased expression:pleuraRGD 
malignant pleural mesothelioma  ISOSLC7A5 (Homo sapiens)151660330; 151660330protein:increased expression:pleuraRGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc7a5  (solute carrier family 7 member 5)

Genes (Mus musculus)
Slc7a5  (solute carrier family 7 (cationic amino acid transporter, y+ system), member 5)

Genes (Homo sapiens)
SLC7A5  (solute carrier family 7 member 5)


Additional Information