RGD Reference Report - An antisense oligodeoxyribonucleotide to m-calpain mRNA inhibits myoblast fusion. - Rat Genome Database

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An antisense oligodeoxyribonucleotide to m-calpain mRNA inhibits myoblast fusion.

Authors: Balcerzak, D  Poussard, S  Brustis, J J  Elamrani, N  Soriano, M  Cottin, P  Ducastaing, A 
Citation: Balcerzak D, etal., J Cell Sci. 1995 May;108 ( Pt 5):2077-82.
RGD ID: 13792659
Pubmed: PMID:7657725   (View Abstract at PubMed)

Previous studies have led to the hypothesis of a possible role for m-calpain (EC 3.4.22.17) in myoblast fusion in culture in vitro. To support this hypothesis, an antisense strategy has been used with cultured primary rat myoblasts. Using an appropriate antisense oligodeoxyribonucleotide to m-calpain mRNA, an inhibition of myoblast fusion has been observed, the maximum being obtained when the cell culture was treated with 30 microM of oligomer. Synthesis of m-calpain was decreased by 48% while high concentrations of antisense oligonucleotide do not significantly affect myoblast proliferation. The specificity of m-calpain intervention during fusion has also been confirmed using antisense oligonucleotides to mu-calpain and p94 mRNAs, respectively.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of myoblast fusion  IMP 13792659 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Capn2  (calpain 2)


Additional Information