RGD Reference Report - [Regulatory effect of IkappaBalpha on NF-kappaB activity during viral gene transactivation in patients with steroid responsive simple nephrotic syndrome].

General

[Regulatory effect of IkappaBalpha on NF-kappaB activity during viral gene transactivation in patients with steroid responsive simple nephrotic syndrome].
Authors:	Tao, Yu-Hong Wang, Zheng Li, Chan-Sheng Zhu, Xiao-Shi
Citation:	Tao YH, etal., Sichuan Da Xue Xue Bao Yi Xue Ban. 2007 Mar;38(2):230-3.
RGD ID:	127285019
Pubmed:	PMID:17441336 (View Abstract at PubMed)
OBJECTIVE: To investigate the expression of inhibitory kappa B alpha (IkappaBalpha) and it's regulatory effect on nuclear factor kappa B (NF-kappaB) during viral gene transactivation triggered by respiratory tract viruses in steroid responsive simple nephrotic syndrome (SRSNS). METHODS: Children with SRSNS (including at active and remissive stage) were examined for this research project, and healthy children were chosen as controls. TaqMan reverse transcription-PCR (RT-PCR), Western blot, electro-mobility shift assays were performed to study the protein expression of IkappaBalpha gene and the activity of NF-kappaB in peripheral blood mononuclear cells (PBMCs). Simultaneously, RT-PCR was used to detect the gene expression of respiratory tract viruses (including respiratory syncytial virus and influenza virus), and viral antibody was measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: Comparing with that from SRSNS at remissive stage and healthy children, the activity of NF-kappaB from SRSNS at active stage was much higher statistically (P < 0.05). The positive correlation between NF-kappaB activation and the gene expression of respiratory tract viruses was observed (OR = 27, Kappa coefficient = 0.59, P <0. 05). In addition, levels of IkappaBalpha protein from children with SRSNS at active stage were much lower than those from healthy children and those from children with SRSNS at remissive stage (P < 0.05). There was a linear negative correlation between the activity of NF-kappaB and the protein level of IkappaBalpha (r = -0.884, P < 0.05). On the other hand, levels of IkappaBalpha mRNA from SRSNS at active stage were much higher than those from healthy children, but much lower than those from children with SRSNS at remissive stage (P < 0.05). CONCLUSION: Abnormal expression of IkappaBalpha, which is attributed to regulate activity of NF-kappaB, occurs during viral gene transactivation triggered by respiratory tract viruses in SRSNS.

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Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
NFKBIA	Human	nephrotic syndrome	exacerbates	IEP		protein:decreased expression:peripheral blood mononuclear cell (human)	RGD
Nfkbia	Rat	nephrotic syndrome	exacerbates	ISO	NFKBIA (Homo sapiens)	protein:decreased expression:peripheral blood mononuclear cell (human)	RGD
Nfkbia	Mouse	nephrotic syndrome	exacerbates	ISO	NFKBIA (Homo sapiens)	protein:decreased expression:peripheral blood mononuclear cell (human)	RGD

Objects Annotated

Genes (Rattus norvegicus)

Nfkbia (NFKB inhibitor alpha)

Genes (Mus musculus)

Nfkbia (nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha)

Genes (Homo sapiens)

NFKBIA (NFKB inhibitor alpha)

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[Regulatory effect of IkappaBalpha on NF-kappaB activity during viral gene transactivation in patients with steroid responsive simple nephrotic syndrome].