RGD Reference Report - Roles of naofen, a novel WD-repeat-2 protein, in the CCl4-treated livers--a possible relationship to cell proliferation. - Rat Genome Database

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Roles of naofen, a novel WD-repeat-2 protein, in the CCl4-treated livers--a possible relationship to cell proliferation.

Authors: An, J  Tsunekawa, K  Feng, GG  Li, C  Huang, L  Ito, Y  Sugiyama, S  Kurokawa, T  Koide, T  Nonami, T  Ishikawa, N 
Citation: An J, etal., Eur J Pharmacol. 2008 Jun 10;587(1-3):285-90. doi: 10.1016/j.ejphar.2008.04.022. Epub 2008 Apr 13.
RGD ID: 11553927
Pubmed: PMID:18472094   (View Abstract at PubMed)
DOI: DOI:10.1016/j.ejphar.2008.04.022   (Journal Full-text)

Naofen (GenBank accession no. EF613262), a newly found intracellular protein in the WD-repeat-2 protein family, has been cloned as an anti-verotoxin II antibody immunoreactive substance, and the nucleotide- and amino acid-sequences have been clarified. The present study was undertaken to evaluate the roles of naofen especially in carbon tetrachloride (CCl4-induced cirrhosis model of rats, also in partial hepatectomy. Naofen mRNA expressions were observed from the early phases of cirrhosis development and during regenerative phases after partial hepatectomy, more remarkable in the former. Naofen immunoreactive fragments located in the vascular endothelial cells and peri-vascular spaces in normal livers especially in Glisson's areas, being strongly stained in the connective tissues 8 weeks after starting CCl4-injections, besides in the cytoplasm of hepatocytes in pseudo-lobules. In contrast, partial hepatectomy caused a small increase of naofen expressions in the whole hepatocytes, and significantly in the endothelial cells of portal veins and hepatic arterioles. Furthermore, in parallel to the degree of naofen mRNA and protein expressions, the rates of double-nuclei cells to total hepatocytes in the Glisson's areas increased in both cirrhosis and partial hepatectomy, suggesting a relationship between naofen expression and mitosis. In in-vitro studies with cell lines, vascular endothelial growth factor, a cell proliferation stimulant, increased the naofen mRNA expressions in HepG(2) cell lines, whereas paclitaxel, a cytotoxic anti-cancer drug, diminished them in NRK52E, both concentration-dependently. These results indicated that naofen immunoreactive fragments play an important role in the cell proliferation, relevant for analyzing the regenerative phases during cirrhosis developments and after partial hepatectomy.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
liver cirrhosis  ISOWdr35 (Rattus norvegicus)11553927; 11553927mRNA:increased expression:liver (rat)RGD 
liver cirrhosis  IEP 11553927mRNA:increased expression:liver (rat)RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to xenobiotic stimulus  IEP 11553927 RGD 
liver regeneration  IEP 11553927 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Wdr35  (WD repeat domain 35)

Genes (Mus musculus)
Wdr35  (WD repeat domain 35)

Genes (Homo sapiens)
WDR35  (WD repeat domain 35)


Additional Information