RGD Reference Report - Repetitive hyperbaric oxygenation attenuates reactive astrogliosis and suppresses expression of inflammatory mediators in the rat model of brain injury. - Rat Genome Database

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Repetitive hyperbaric oxygenation attenuates reactive astrogliosis and suppresses expression of inflammatory mediators in the rat model of brain injury.

Authors: Lavrnja, I  Parabucki, A  Brkic, P  Jovanovic, T  Dacic, S  Savic, D  Pantic, I  Stojiljkovic, M  Pekovic, S 
Citation: Lavrnja I, etal., Mediators Inflamm. 2015;2015:498405. doi: 10.1155/2015/498405. Epub 2015 Apr 20.
RGD ID: 11344972
Pubmed: PMID:25972624   (View Abstract at PubMed)
PMCID: PMC4417949   (View Article at PubMed Central)
DOI: DOI:10.1155/2015/498405   (Journal Full-text)

The exact mechanisms by which treatment with hyperbaric oxygen (HBOT) exerts its beneficial effects on recovery after brain injury are still unrevealed. Therefore, in this study we investigated the influence of repetitive HBOT on the reactive astrogliosis and expression of mediators of inflammation after cortical stab injury (CSI). CSI was performed on male Wistar rats, divided into control, sham, and lesioned groups with appropriate HBO. The HBOT protocol was as follows: 10 minutes of slow compression, 2.5 atmospheres absolute (ATA) for 60 minutes, and 10 minutes of slow decompression, once a day for 10 consecutive days. Data obtained using real-time polymerase chain reaction, Western blot, and immunohistochemical and immunofluorescence analyses revealed that repetitive HBOT applied after the CSI attenuates reactive astrogliosis and glial scarring, and reduces expression of GFAP (glial fibrillary acidic protein), vimentin, and ICAM-1 (intercellular adhesion molecule-1) both at gene and tissue levels. In addition, HBOT prevents expression of CD40 and its ligand CD40L on microglia, neutrophils, cortical neurons, and reactive astrocytes. Accordingly, repetitive HBOT, by prevention of glial scarring and limiting of expression of inflammatory mediators, supports formation of more permissive environment for repair and regeneration.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Brain Injuries treatmentISOCd40lg (Rattus norvegicus)11344972; 11344972 RGD 
Brain Injuries treatmentIDA 11344972 RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cell body  IDA 11344972 RGD 
cell projection  IDA 11344972 RGD 
neuronal cell body  IDA 11344972 RGD 
varicosity  IDA 11344972 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cd40  (CD40 molecule)
Cd40lg  (CD40 ligand)

Genes (Mus musculus)
Cd40lg  (CD40 ligand)

Genes (Homo sapiens)
CD40LG  (CD40 ligand)


Additional Information