RGD Reference Report - ABC transporter function and regulation at the blood-spinal cord barrier. - Rat Genome Database

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ABC transporter function and regulation at the blood-spinal cord barrier.

Authors: Campos, CR  Schroter, C  Wang, X  Miller, DS 
Citation: Campos CR, etal., J Cereb Blood Flow Metab. 2012 Aug;32(8):1559-66. doi: 10.1038/jcbfm.2012.47. Epub 2012 Apr 4.
RGD ID: 11099986
Pubmed: PMID:22472606   (View Abstract at PubMed)
PMCID: PMC3421094   (View Article at PubMed Central)
DOI: DOI:10.1038/jcbfm.2012.47   (Journal Full-text)

We present here an initial characterization of ATP binding cassette (ABC) transporter function and regulation at the blood-spinal cord barrier. We isolated capillaries from rat spinal cords and studied transport function using a confocal microscopy-based assay and protein expression using western blots. These capillaries exhibited transport function and protein expression of P-glycoprotein (Abcb1), multidrug resistance protein 2 (Mrp2, Abcc2), and breast cancer-related protein (Bcrp, Abcg2). Exposing isolated capillaries to dioxin (activates aryl hydrocarbon receptor) increased transport mediated by all three transporters. Brain and spinal cord capillaries from dioxin-dosed rats exhibited increased P-glycoprotein-mediated transport and increased protein expression for all three ABC transporters. These findings indicate similar ABC transporter expression, function, and regulation at the blood-spinal cord and blood-brain barriers.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to 2,3,7,8-tetrachlorodibenzodioxine  IEP 11099986 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Abcg2  (ATP binding cassette subfamily G member 2)


Additional Information