RGD Reference Report - A glucose-6-phosphate isomerase peptide induces T and B cell-dependent chronic arthritis in C57BL/10 mice: arthritis without reactive oxygen species and complement.

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A glucose-6-phosphate isomerase peptide induces T and B cell-dependent chronic arthritis in C57BL/10 mice: arthritis without reactive oxygen species and complement.
Authors:	Pizzolla, A Wing, K Holmdahl, R
Citation:	Pizzolla A, etal., Am J Pathol. 2013 Oct;183(4):1144-55. doi: 10.1016/j.ajpath.2013.06.019. Epub 2013 Jul 30.
RGD ID:	11051957
Pubmed:	PMID:23911657 (View Abstract at PubMed)
DOI:	DOI:10.1016/j.ajpath.2013.06.019 (Journal Full-text)
Immunization with human glucose-6-phosphate isomerase (hG6PI) protein or with several of its peptides induces arthritis in DBA/1 mice. We investigated G6PI peptide-induced arthritis in C57BL/10 mice and the effect of oxidative burst on disease. To study the arthritogenicity of G6PI peptides and its immune dependency, we used genetically modified and congenic mice on the C57BL/10 background and in vitro T- and B-cell assays. hG6PI(325-339) peptide induced arthritis in C57BL/10 mice. The disease was associated with major histocompatibility complex class II and was dependent on T cells, B cells, and complement C5. Th1 and Th17 cells primed with the hG6PI(325-339) peptide cross-reacted with the murine G6PI protein. The severity of the disease increased in mice carrying a mutation in Ncf1 (Ncf1/), which abolishes the NADPH oxidase 2 complex oxidative burst. Ncf1/ mice developed arthritis also on immunization with the mouse G6PI325-339 peptide and in the absence of C5. The antibody responses to the G6PI protein and peptides were minimal in both Ncf1/ and wild-type mice. Herein is described G6PI peptide as the first peptide to induce arthritis in C57BL/10 mice. The differences between the wild-type and Ncf1/ mice suggest that an alternative complement-independent arthritogenic pathway could be operative in the absence of oxidative burst.

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Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
GPI	Human	Experimental Arthritis		IDA			RGD
Gpi	Rat	Experimental Arthritis		ISO	GPI (Homo sapiens)		RGD
Gpi1	Mouse	Experimental Arthritis		ISO	GPI (Homo sapiens)		RGD

Objects Annotated

Genes (Rattus norvegicus)

Gpi (glucose-6-phosphate isomerase)

Genes (Mus musculus)

Gpi1 (glucose-6-phosphate isomerase 1)

Genes (Homo sapiens)

GPI (glucose-6-phosphate isomerase)

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A glucose-6-phosphate isomerase peptide induces T and B cell-dependent chronic arthritis in C57BL/10 mice: arthritis without reactive oxygen species and complement.