RGD Reference Report - The Q-Soluble-N-Ethylmaleimide-Sensitive Factor Attachment Protein Receptor (Q-SNARE) SNAP-47 Regulates Trafficking of Selected Vesicle-Associated Membrane Proteins (VAMPs). - Rat Genome Database

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The Q-Soluble-N-Ethylmaleimide-Sensitive Factor Attachment Protein Receptor (Q-SNARE) SNAP-47 Regulates Trafficking of Selected Vesicle-Associated Membrane Proteins (VAMPs).

Authors: Kuster, A  Nola, S  Dingli, F  Vacca, B  Gauchy, C  Beaujouan, JC  Nunez, M  Moncion, T  Loew, D  Formstecher, E  Galli, T  Proux-Gillardeaux, V 
Citation: Kuster A, etal., J Biol Chem. 2015 Sep 10. pii: jbc.M115.666362.
RGD ID: 10412295
Pubmed: PMID:26359495   (View Abstract at PubMed)
PMCID: PMC4653666   (View Article at PubMed Central)
DOI: DOI:10.1074/jbc.M115.666362   (Journal Full-text)

SNAREs constitute the core machinery of intracellular membrane fusion but vesicular SNAREs localize to specific compartments via largely unknown mechanisms. Here we identified an interaction between VAMP7 and SNAP-47 using a proteomics approach. We found that SNAP-47 mainly localized to cytoplasm, the endoplasmic reticulum and ERGIC and could also shuttle between the cytoplasm and the nucleus. SNAP-47 preferentially interacted with TGN VAMP4 and post-Golgi VAMPs 7 and 8. SNAP-47 also interacted with ER and Golgi Syntaxin 5 and with Syntaxin 1 in the absence of Munc18a, when Syntaxin 1 is retained in the ER. A C-terminally truncated SNAP-47 was impaired in interaction with VAMPs and affected their subcellular distribution. SNAP-47 silencing further shifted the subcellular localization of VAMP4 from the Golgi apparatus to the ER. WT and mutant SNAP-47 overexpression impaired VAMP7 exocytic activity. We conclude that SNAP-47 plays a role in the proper localization and function of a subset of VAMPs likely via regulation of their transport through the early secretory pathway.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein binding enablesIPIUniProtKB:P6176510412295PMID:26359495IntAct 
protein binding enablesIPIUniProtKB:Q5SQN110412295PMID:26359495IntAct 
protein binding enablesIPIUniProtKB:P3285110412295PMID:26359495IntAct 

Objects Annotated

Genes (Rattus norvegicus)
Stx1a  (syntaxin 1A)
Stxbp1  (syntaxin binding protein 1)


Additional Information