RGD Reference Report - [Roles of periostin in proliferation and migration of vascular smooth muscle cells and the effect of atorvastatin on them]. - Rat Genome Database

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[Roles of periostin in proliferation and migration of vascular smooth muscle cells and the effect of atorvastatin on them].

Authors: Li, Jingle  Yan, Wenguang  Wang, Jiangang  Tan, Wenpeng  Zhou, Yu  Yang, Kan 
Citation: Li J, etal., Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2012 Jul;37(7):689-94. doi: 10.3969/j.issn.1672-7347.2012.07.007.
RGD ID: 10040993
Pubmed: PMID:22886209   (View Abstract at PubMed)
DOI: DOI:10.3969/j.issn.1672-7347.2012.07.007   (Journal Full-text)


OBJECTIVE: To investigate the expression of periostin in in vitro cultured vascular smooth muscle cells (VSMCs) induced by TGF-ß1 and the relationship between periostin expression and the migration and proliferation of the VSMCs. Further, to investigate the effects of atorvastatin on the above-mentioned processes and the molecular mechanisms of atorvastatin inhibition of TGF-ß1- induced periostin production.
METHODS: Rat aorta smooth muscle cells were cultivated by the method of tissue explants adherence. Cells of generation 3 to 6 were used as the experimental system. Primary cultured rat vascular smooth muscle cells were treated by TGF-ß1 and different concentrations of atorvastatin,Y-2763 (Rho kinase inhibitor), or atorvastatin plus MVA for 24 hours. The expression of periostin was measured by RT-PCR and Western blot. A Boyden chamber assay was used to measure cell migration, and an MTT test was used to measure cell proliferation.
RESULTS: Periostin expression in rat VSMCs stimulated by TGF-ß1 increased significantly (4.158 ± 0.515 vs 0.385 ± 0.031), VSMC migration(25 ± 4 vs 8 ± 2) and proliferation (0.85 ± 0.06 vs 0.32 ± 0.03) also increased significantly. Atorvastatin significantly inhibited TGF-ß1-induced periostin production in rat VSMCs, as well as VSMC migration and proliferation, in a dose-dependent manner. Rho kinase inhibitor Y-27632 significantly inhibited TGF-ß1-induced periostin production in rat VSMCs (2.082 ± 0.245). The inhibitory effect of atorvastatin on periostin upregulation induced by TGF-ß1 was reversed by mevalonate (3.838 ± 0.326).
CONCLUSION: Periostin can promote rat VSMC migration and proliferation. Atorvastatin inhibition of periostin expression induced by TGF-ß1 in VSMCs may be exerted by inhibition of the production of MVA and other isoprene compounds and by blocking the Rho/Rho kinase signaling pathway.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to transforming growth factor beta stimulus  IEP 10040993 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Postn  (periostin)


Additional Information