Saxo1 (stabilizer of axonemal microtubules 1) - Rat Genome Database

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Pathways
Gene: Saxo1 (stabilizer of axonemal microtubules 1) Rattus norvegicus
Analyze
Symbol: Saxo1
Name: stabilizer of axonemal microtubules 1
RGD ID: 1583685
Description: Predicted to enable microtubule binding activity. Predicted to be involved in several processes, including cold acclimation; positive regulation of cilium assembly; and protein stabilization. Predicted to be located in motile cilium. Predicted to be active in microtubule cytoskeleton and sperm flagellum. Orthologous to human SAXO1 (stabilizer of axonemal microtubules 1); INTERACTS WITH acrylamide; atrazine; bisphenol A.
Type: protein-coding
RefSeq Status: VALIDATED
Previously known as: Fam154a; family with sequence similarity 154, member A; hypothetical protein LOC679802; LOC679802; rCG55202-like; uncharacterized protein LOC679802
RGD Orthologs
Human
Mouse
Chinchilla
Bonobo
Dog
Squirrel
Pig
Green Monkey
Naked Mole-Rat
Alliance Orthologs
More Info more info ...
Latest Assembly: GRCr8 - GRCr8 Assembly
Position:
Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCr85105,982,949 - 106,139,298 (-)NCBIGRCr8GRCr8GRCr8
GRCr8 Ensembl5105,982,322 - 106,143,859 (-)EnsemblGRCr8
mRatBN7.25100,937,134 - 101,093,308 (-)NCBImRatBN7.2mRatBN7.2
mRatBN7.2 Ensembl5100,937,130 - 101,093,281 (-)EnsemblmRatBN7.2
Rnor_6.05104,771,081 - 104,921,001 (-)NCBIRnor_6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 Ensembl5104,771,392 - 104,920,906 (-)Ensemblrn6Rnor6.0
Rnor_5.05108,756,769 - 108,908,811 (-)NCBIRnor_5.0Rnor_5.0rn5
RGSC_v3.45105,443,094 - 105,589,653 (-)NCBIRGSC_v3.4RGSC_v3.4rn4
Celera599,430,281 - 99,578,228 (-)NCBICelera
Cytogenetic Map5q31NCBI
JBrowse:




Gene-Chemical Interaction Annotations     Click to see Annotation Detail View
Gene Ontology Annotations     Click to see Annotation Detail View

Biological Process

Cellular Component

Molecular Function
microtubule binding  (IBA,IEA,ISO)
protein binding  (ISO)

References

References - curated
# Reference Title Reference Citation
1. Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Gaudet P, etal., Brief Bioinform. 2011 Sep;12(5):449-62. doi: 10.1093/bib/bbr042. Epub 2011 Aug 27.
2. Data Import for Chemical-Gene Interactions RGD automated import pipeline for gene-chemical interactions
Additional References at PubMed
PMID:25673876  


Genomics

Comparative Map Data
Saxo1
(Rattus norvegicus - Norway rat)
Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCr85105,982,949 - 106,139,298 (-)NCBIGRCr8GRCr8GRCr8
GRCr8 Ensembl5105,982,322 - 106,143,859 (-)EnsemblGRCr8
mRatBN7.25100,937,134 - 101,093,308 (-)NCBImRatBN7.2mRatBN7.2
mRatBN7.2 Ensembl5100,937,130 - 101,093,281 (-)EnsemblmRatBN7.2
Rnor_6.05104,771,081 - 104,921,001 (-)NCBIRnor_6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 Ensembl5104,771,392 - 104,920,906 (-)Ensemblrn6Rnor6.0
Rnor_5.05108,756,769 - 108,908,811 (-)NCBIRnor_5.0Rnor_5.0rn5
RGSC_v3.45105,443,094 - 105,589,653 (-)NCBIRGSC_v3.4RGSC_v3.4rn4
Celera599,430,281 - 99,578,228 (-)NCBICelera
Cytogenetic Map5q31NCBI
SAXO1
(Homo sapiens - human)
Human AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCh38918,927,650 - 19,049,339 (-)NCBIGRCh38GRCh38hg38GRCh38
GRCh38.p14 Ensembl918,858,906 - 19,049,358 (-)Ensemblhg38GRCh38
GRCh37918,927,648 - 19,049,337 (-)NCBIGRCh37GRCh37hg19GRCh37
Build 36918,917,893 - 19,023,184 (-)NCBIBuild 36Build 36hg18NCBI36
Build 34918,917,892 - 19,023,184NCBI
Celera918,854,088 - 18,959,450 (-)NCBICelera
Cytogenetic Map9p22.1NCBI
HuRef918,889,549 - 18,994,879 (-)NCBIHuRef
CHM1_1918,927,885 - 19,033,250 (-)NCBICHM1_1
T2T-CHM13v2.0918,940,631 - 19,062,204 (-)NCBIT2T-CHM13v2.0
Saxo1
(Mus musculus - house mouse)
Mouse AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCm39486,362,662 - 86,476,540 (-)NCBIGRCm39GRCm39mm39
GRCm39 Ensembl486,362,878 - 86,476,565 (-)EnsemblGRCm39 EnsemblGRCm39
GRCm38486,444,425 - 86,558,303 (-)NCBIGRCm38GRCm38mm10GRCm38
GRCm38.p6 Ensembl486,444,641 - 86,558,328 (-)Ensemblmm10GRCm38
MGSCv37486,090,602 - 86,204,207 (-)NCBIMGSCv37MGSCv37mm9NCBIm37
MGSCv36485,915,929 - 86,029,561 (-)NCBIMGSCv36mm8
Celera484,960,048 - 85,073,131 (-)NCBICelera
Cytogenetic Map4C4NCBI
cM Map440.69NCBI
Saxo1
(Chinchilla lanigera - long-tailed chinchilla)
Chinchilla AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
ChiLan1.0 EnsemblNW_00495543421,414,033 - 21,494,399 (-)Ensembl
ChiLan1.0NW_00495543421,414,937 - 21,454,901 (-)NCBIChiLan1.0ChiLan1.0
SAXO1
(Pan paniscus - bonobo/pygmy chimpanzee)
Bonobo AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
NHGRI_mPanPan1-v211105,503,056 - 105,740,158 (+)NCBINHGRI_mPanPan1-v2
NHGRI_mPanPan19105,509,000 - 105,620,800 (+)NCBINHGRI_mPanPan1
Mhudiblu_PPA_v0918,741,406 - 18,868,726 (-)NCBIMhudiblu_PPA_v0Mhudiblu_PPA_v0panPan3
PanPan1.1919,234,977 - 19,346,886 (-)NCBIPanPan1.1PanPan1.1panPan2
PanPan1.1 Ensembl919,234,977 - 19,346,886 (-)EnsemblpanPan2panpan1.1
SAXO1
(Canis lupus familiaris - dog)
Dog AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
CanFam3.11138,771,201 - 38,873,442 (-)NCBICanFam3.1CanFam3.1canFam3CanFam3.1
CanFam3.1 Ensembl1138,771,209 - 38,871,709 (-)EnsemblcanFam3CanFam3.1
Dog10K_Boxer_Tasha1137,430,159 - 37,532,190 (-)NCBIDog10K_Boxer_TashaDog10K_Boxer_Tasha
ROS_Cfam_1.01139,704,270 - 39,806,475 (-)NCBIROS_Cfam_1.0ROS_Cfam_1.0
ROS_Cfam_1.0 Ensembl1139,704,233 - 39,805,060 (-)EnsemblROS_Cfam_1.0 Ensembl
UMICH_Zoey_3.11138,397,934 - 38,499,754 (-)NCBIUMICH_Zoey_3.1
UNSW_CanFamBas_1.01138,192,582 - 38,294,769 (-)NCBIUNSW_CanFamBas_1.0
UU_Cfam_GSD_1.01138,829,428 - 38,931,733 (-)NCBIUU_Cfam_GSD_1.0UU_Cfam_GSD_1.0
Saxo1
(Ictidomys tridecemlineatus - thirteen-lined ground squirrel)
Squirrel AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
HiC_Itri_2NW_024404947153,215,697 - 153,313,168 (-)NCBIHiC_Itri_2
SpeTri2.0NW_004936652885,616 - 915,113 (-)NCBISpeTri2.0SpeTri2.0SpeTri2.0
SAXO1
(Sus scrofa - pig)
Pig AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
Sscrofa11.1 Ensembl1203,788,341 - 203,923,516 (+)EnsemblsusScr11Sscrofa11.1
Sscrofa11.11203,788,322 - 203,923,889 (+)NCBISscrofa11.1Sscrofa11.1susScr11Sscrofa11.1
SAXO1
(Chlorocebus sabaeus - green monkey)
Green Monkey AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
ChlSab1.11260,671,508 - 60,788,923 (+)NCBIChlSab1.1ChlSab1.1chlSab2
ChlSab1.1 Ensembl1260,766,059 - 60,788,518 (+)EnsemblChlSab1.1 EnsemblchlSab2Vervet-AGM
Vero_WHO_p1.0NW_02366603848,796,820 - 48,914,658 (+)NCBIVero_WHO_p1.0Vero_WHO_p1.0
Saxo1
(Heterocephalus glaber - naked mole-rat)
Naked Mole-Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
HetGla_female_1.0 EnsemblNW_00462473622,926,686 - 22,958,800 (-)EnsemblHetGla_female_1.0 EnsemblhetGla2HetGla_female_1.0 Ensembl
HetGla 1.0NW_00462473622,926,420 - 23,035,788 (-)NCBIHetGla 1.0HetGla 1.0hetGla2

Variants

.
Variants in Saxo1
635 total Variants
miRNA Target Status (No longer updated)

Predicted Target Of
Summary Value
Count of predictions:163
Count of miRNA genes:117
Interacting mature miRNAs:131
Transcripts:ENSRNOT00000037047, ENSRNOT00000071318
Prediction methods:Microtar, Miranda, Pita, Rnahybrid, Targetscan
Result types:miRGate_prediction

The detailed report is available here: Full Report CSV TAB Printer

miRNA Target Status data imported from miRGate (http://mirgate.bioinfo.cnio.es/).
For more information about miRGate, see PMID:25858286 or access the full paper here.


QTLs in Region (GRCr8)
The following QTLs overlap with this region.    Full Report CSV TAB Printer Gviewer
RGD IDSymbolNameLODP ValueTraitSub TraitChrStartStopSpecies
1331796Thshl2Thyroid stimulating hormone level QTL 22.3blood thyroid-stimulating hormone amount (VT:0005119)serum thyroid stimulating hormone level (CMO:0001248)5102105698152749382Rat
1357396Bw44Body weight QTL 444.19body mass (VT:0001259)body weight (CMO:0000012)573779642118779642Rat
2303586Gluco52Glucose level QTL 522blood glucose amount (VT:0000188)blood glucose level (CMO:0000046)5100826632145826632Rat
1549845Scl44Serum cholesterol level QTL 446blood cholesterol amount (VT:0000180)serum total cholesterol level (CMO:0000363)544924861153890773Rat
70212Niddm25Non-insulin dependent diabetes mellitus QTL 253.54blood glucose amount (VT:0000188)blood glucose level (CMO:0000046)538207390136631402Rat
1298070Scl18Serum cholesterol level QTL 183.7blood LDL cholesterol amount (VT:0000181)calculated plasma low density lipoprotein cholesterol level (CMO:0001245)584630798129630798Rat
61452Ciaa5CIA Autoantibody QTL 53.5blood autoantibody amount (VT:0003725)calculated serum anti-rat type 2 collagen autoantibody titer (CMO:0001281)599905133172190305Rat
1331801Rf33Renal function QTL 334.149kidney blood vessel physiology trait (VT:0100012)absolute change in renal vascular resistance (CMO:0001900)548523123134369373Rat
2302381Bw84Body weight QTL 844.47body mass (VT:0001259)body mass index (BMI) (CMO:0000105)573779642118779642Rat
1357402Bw46Body weight QTL 464.47body mass (VT:0001259)body mass index (BMI) (CMO:0000105)573779642118779642Rat
7411582Foco3Food consumption QTL 37.50.001eating behavior trait (VT:0001431)feed conversion ratio (CMO:0001312)592583776137583776Rat
61393Bp7Blood pressure QTL 74.50.0001arterial blood pressure trait (VT:2000000)systolic blood pressure (CMO:0000004)565089051166764498Rat
1598859Cm66Cardiac mass QTL 662heart mass (VT:0007028)heart weight to body weight ratio (CMO:0000074)584630798129630798Rat
1578766Tcas11Tongue tumor susceptibility QTL 114.12tongue integrity trait (VT:0010553)number of squamous cell tumors of the tongue with diameter greater than 3 mm (CMO:0001950)551657967166600247Rat
1582212Livw2Liver weight QTL 23.50.0004liver mass (VT:0003402)liver weight to body weight ratio (CMO:0000633)5104018576124314782Rat
7411564Bw135Body weight QTL 1350.001body mass (VT:0001259)body weight gain (CMO:0000420)592583776137583776Rat
8657050Bw146Body weight QTL 14619.840.001body mass (VT:0001259)body weight gain (CMO:0000420)598972960143972960Rat
1576312Emca8Estrogen-induced mammary cancer QTL 84.1mammary gland integrity trait (VT:0010552)mammary tumor number (CMO:0000343)555124766172190305Rat
7411601Foco12Food consumption QTL 1219.70.001eating behavior trait (VT:0001431)feed conversion ratio (CMO:0001312)592583776137583776Rat
2303618Vencon1Ventilatory control QTL 13.8respiration trait (VT:0001943)oxygen consumption (CMO:0002169)5100826632145826632Rat
7394708Emca11Estrogen-induced mammary cancer QTL 11mammary gland integrity trait (VT:0010552)mammary tumor number (CMO:0000343)573176602118176602Rat
1598846Bp293Blood pressure QTL 2933.9arterial blood pressure trait (VT:2000000)systolic blood pressure (CMO:0000004)584630798129630798Rat
7394710Emca12Estrogen-induced mammary cancer QTL 12mammary gland integrity trait (VT:0010552)mammary tumor number (CMO:0000343)594034903139034903Rat
1578673Bmd13Bone mineral density QTL 134.9femur mineral mass (VT:0010011)trabecular volumetric bone mineral density (CMO:0001729)569474415114474415Rat
1358909Kidm25Kidney mass QTL 251.87kidney mass (VT:0002707)both kidneys wet weight to body weight ratio (CMO:0000340)595062526133270832Rat
70189Mcs5Mammary carcinoma susceptibility QTL 510.51mammary gland integrity trait (VT:0010552)mammary tumor number (CMO:0000343)560601597137492949Rat
1600362Mcs19Mammary carcinoma susceptibility QTL 192.5mammary gland integrity trait (VT:0010552)mammary tumor number (CMO:0000343)582032155127032155Rat
1354598Srn6Serum renin concentration QTL 63.8blood renin amount (VT:0003349)plasma renin activity level (CMO:0000116)574335621156302135Rat
2290448Scl54Serum cholesterol level QTL 542.93blood cholesterol amount (VT:0000180)plasma total cholesterol level (CMO:0000585)536460764136631402Rat
61426Scl2Serum cholesterol level QTL 27.30.001blood cholesterol amount (VT:0000180)serum total cholesterol level (CMO:0000363)564589016109589016Rat
1298086Bp156Blood pressure QTL 156arterial blood pressure trait (VT:2000000)systolic blood pressure (CMO:0000004)589369373134369373Rat
2290005Mcs24Mammary carcinoma susceptibility QTL 24mammary gland integrity trait (VT:0010552)mammary tumor number (CMO:0000343)569835120114835120Rat
2306971Anxrr21Anxiety related response QTL 219.47fear/anxiety-related behavior trait (VT:1000241)number of entries into a discrete space in an experimental apparatus (CMO:0000960)570969494136328156Rat
1358895Bp254Blood pressure QTL 2543.60.0003arterial blood pressure trait (VT:2000000)mean arterial blood pressure (CMO:0000009)597492524133270832Rat
2312671Scl64Serum cholesterol level QTL 640.001blood cholesterol amount (VT:0000180)serum total cholesterol level (CMO:0000363)573779642118779642Rat
1358889Bp261Blood pressure QTL 2612.86arterial blood pressure trait (VT:2000000)mean arterial blood pressure (CMO:0000009)595062526133270832Rat
6903316Bw113Body weight QTL 11320.0103body mass (VT:0001259)body weight (CMO:0000012)592881678137881678Rat
1358187Emca1Estrogen-induced mammary cancer QTL 14.4mammary gland integrity trait (VT:0010552)post-insult time to mammary tumor formation (CMO:0000345)5104262817153890625Rat

Markers in Region
D5Got131  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.25101,112,265 - 101,112,414 (+)MAPPERmRatBN7.2
Rnor_6.05104,939,992 - 104,940,140NCBIRnor6.0
Rnor_5.05108,927,799 - 108,927,947UniSTSRnor5.0
RGSC_v3.45105,658,583 - 105,658,732RGDRGSC3.4
RGSC_v3.45105,658,584 - 105,658,732UniSTSRGSC3.4
Celera599,597,007 - 99,597,155UniSTS
RGSC_v3.15105,663,810 - 105,663,958RGD
RH 3.4 Map5741.8UniSTS
RH 3.4 Map5741.8RGD
RH 2.0 Map5660.2RGD
Cytogenetic Map5q32UniSTS
BE107919  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.25100,937,532 - 100,937,742 (+)MAPPERmRatBN7.2
Rnor_6.05104,771,616 - 104,771,825NCBIRnor6.0
Rnor_5.05108,757,350 - 108,757,559UniSTSRnor5.0
RGSC_v3.45105,443,460 - 105,443,669UniSTSRGSC3.4
Celera599,430,816 - 99,431,025UniSTS
RH 3.4 Map5735.3UniSTS
Cytogenetic Map5q32UniSTS
RH140944  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.25101,114,607 - 101,114,788 (+)MAPPERmRatBN7.2
Rnor_6.05104,942,334 - 104,942,514NCBIRnor6.0
Rnor_5.05108,930,141 - 108,930,321UniSTSRnor5.0
RGSC_v3.45105,660,926 - 105,661,106UniSTSRGSC3.4
Celera599,599,349 - 99,599,529UniSTS
RH 3.4 Map5735.3UniSTS
Cytogenetic Map5q32UniSTS


Expression

RNA-SEQ Expression

appendage
circulatory system
ectoderm
endocrine system
endoderm
exocrine system
hemolymphoid system
hepatobiliary system
integumental system
mesenchyme
mesoderm
nervous system
renal system
reproductive system
respiratory system
sensory system
visual system
3 5 35 27 17 6 12 6 4 52 31 21 12 63 11 1 1

Sequence


Ensembl Acc Id: ENSRNOT00000071318   ⟹   ENSRNOP00000065058
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr8 Ensembl5105,982,949 - 106,129,644 (-)Ensembl
mRatBN7.2 Ensembl5100,937,130 - 101,093,281 (-)Ensembl
Rnor_6.0 Ensembl5104,771,392 - 104,920,906 (-)Ensembl
Ensembl Acc Id: ENSRNOT00000125538   ⟹   ENSRNOP00000107496
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr8 Ensembl5105,982,322 - 106,143,859 (-)Ensembl
RefSeq Acc Id: XM_006238373   ⟹   XP_006238435
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr85105,982,949 - 106,139,298 (-)NCBI
mRatBN7.25100,937,134 - 101,093,308 (-)NCBI
Rnor_6.05104,771,081 - 104,921,001 (-)NCBI
Rnor_5.05108,756,769 - 108,908,811 (-)NCBI
Sequence:
Protein Sequences
Protein RefSeqs NP_001418182 (Get FASTA)   NCBI Sequence Viewer  
  XP_006238435 (Get FASTA)   NCBI Sequence Viewer  
GenBank Protein EDM10444 (Get FASTA)   NCBI Sequence Viewer  
Ensembl Protein ENSRNOP00000065058.3
  ENSRNOP00000107496
  ENSRNOP00000107496.1
RefSeq Acc Id: XP_006238435   ⟸   XM_006238373
- Sequence:
Ensembl Acc Id: ENSRNOP00000065058   ⟸   ENSRNOT00000071318
Ensembl Acc Id: ENSRNOP00000107496   ⟸   ENSRNOT00000125538

Protein Structures
Name Modeler Protein Id AA Range Protein Structure
AF-M0R6K6-F1-model_v2 AlphaFold M0R6K6 1-476 view protein structure

Transcriptome

eQTL   View at Phenogen
WGCNA   View at Phenogen
Tissue/Strain Expression   View at Phenogen

Promoters
RGD ID:13693828
Promoter ID:EPDNEW_R4348
Type:initiation region
Name:Saxo1_1
Description:stabilizer of axonemal microtubules 1
SO ACC ID:SO:0000170
Source:EPDNEW (Eukaryotic Promoter Database, http://epd.vital-it.ch/)
Experiment Methods:Single-end sequencing.
Position:
Rat AssemblyChrPosition (strand)Source
Rnor_6.05104,920,989 - 104,921,049EPDNEW

Additional Information

Database Acc Id Source(s)
AGR Gene RGD:1583685 AgrOrtholog
BioCyc Gene G2FUF-40851 BioCyc
Ensembl Genes ENSRNOG00000048197 Ensembl, ENTREZGENE, UniProtKB/TrEMBL
Ensembl Transcript ENSRNOT00000071318.4 UniProtKB/TrEMBL
  ENSRNOT00000125538 ENTREZGENE
  ENSRNOT00000125538.1 UniProtKB/TrEMBL
InterPro SAXO1/2 UniProtKB/TrEMBL
KEGG Report rno:679802 UniProtKB/TrEMBL
NCBI Gene 679802 ENTREZGENE
PANTHER PTHR31516 UniProtKB/TrEMBL
  STABILIZER OF AXONEMAL MICROTUBULES 1 UniProtKB/TrEMBL
Pfam STOP UniProtKB/TrEMBL
PhenoGen Saxo1 PhenoGen
RatGTEx ENSRNOG00000048197 RatGTEx
UniProt A0ABK0LRW7_RAT UniProtKB/TrEMBL
  A6J863 ENTREZGENE, UniProtKB/TrEMBL
  A6J864 ENTREZGENE, UniProtKB/TrEMBL
  M0R6K6 ENTREZGENE, UniProtKB/TrEMBL


Nomenclature History
Date Current Symbol Current Name Previous Symbol Previous Name Description Reference Status
2017-03-10 Saxo1  stabilizer of axonemal microtubules 1  Fam154a  family with sequence similarity 154, member A  Name and Symbol changed 629549 APPROVED
2008-03-11 Fam154a  family with sequence similarity 154, member A  LOC679802  hypothetical protein LOC679802  Nomenclature updated to reflect human and mouse nomenclature 1299863 APPROVED
2008-01-09 LOC679802  hypothetical protein LOC679802  LOC684579  hypothetical protein LOC684579  Data merged from RGD:1589106 1643240 APPROVED
2006-11-19 LOC679802  hypothetical protein LOC679802      Symbol and Name status set to provisional 70820 PROVISIONAL
2006-11-19 LOC684579  hypothetical protein LOC684579      Symbol and Name status set to provisional 70820 PROVISIONAL