Ust (uronyl-2-sulfotransferase) - Rat Genome Database

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Gene: Ust (uronyl-2-sulfotransferase) Rattus norvegicus
Analyze
Symbol: Ust
Name: uronyl-2-sulfotransferase
RGD ID: 1304743
Description: Predicted to enable 3'-phosphoadenosine 5'-phosphosulfate binding activity; chondroitin 2-sulfotransferase activity; and dermatan 2-sulfotransferase activity. Predicted to be involved in dermatan sulfate proteoglycan biosynthetic process. Predicted to act upstream of or within establishment of cell polarity and regulation of axonogenesis. Predicted to be located in Golgi membrane. Human ortholog(s) of this gene implicated in attention deficit hyperactivity disorder. Orthologous to human UST (uronyl 2-sulfotransferase); PARTICIPATES IN chondroitin sulfate biosynthetic pathway; INTERACTS WITH 2,3,7,8-tetrachlorodibenzodioxine; 2,3,7,8-Tetrachlorodibenzofuran; 6-propyl-2-thiouracil.
Type: protein-coding
RefSeq Status: PROVISIONAL
Previously known as: LOC361450; uronyl 2-sulfotransferase
RGD Orthologs
Human
Mouse
Chinchilla
Bonobo
Dog
Squirrel
Pig
Green Monkey
Naked Mole-Rat
Black Rat
Alliance Orthologs
More Info more info ...
Latest Assembly: GRCr8 - GRCr8 Assembly
Position:
Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCr814,487,316 - 4,782,579 (-)NCBIGRCr8GRCr8GRCr8
GRCr8 Ensembl14,489,925 - 4,782,356 (-)EnsemblGRCr8
mRatBN7.212,666,983 - 2,962,276 (-)NCBImRatBN7.2mRatBN7.2
mRatBN7.2 Ensembl12,669,592 - 2,962,044 (-)EnsemblmRatBN7.2
UTH_Rnor_SHR_Utx12,325,889 - 2,623,315 (-)NCBIUTH_Rnor_SHR_UtxUTH_Rnor_SHR_Utx
UTH_Rnor_SHRSP_BbbUtx_1.018,326,290 - 8,623,726 (-)NCBIUTH_Rnor_SHRSP_BbbUtx_1.0UTH_Rnor_SHRSP_BbbUtx_1.0
UTH_Rnor_WKY_Bbb_1.012,608,001 - 2,905,423 (-)NCBIUTH_Rnor_WKY_Bbb_1.0UTH_Rnor_WKY_Bbb_1.0
Rnor_6.012,330,631 - 2,627,654 (-)NCBIRnor_6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 Ensembl12,333,778 - 2,627,747 (-)Ensemblrn6Rnor6.0
Rnor_5.014,022,062 - 4,313,796 (-)NCBIRnor_5.0Rnor_5.0rn5
RGSC_v3.412,868,196 - 3,158,231 (-)NCBIRGSC_v3.4RGSC_v3.4rn4
Celera11,217,279 - 1,507,424 (-)NCBICelera
RGSC_v3.112,868,195 - 3,158,231 (-)NCBI
Cytogenetic Map1p13NCBI
JBrowse:




Disease Annotations     Click to see Annotation Detail View

Gene Ontology Annotations     Click to see Annotation Detail View

Biological Process

Cellular Component
Golgi membrane  (IEA)
membrane  (IEA)

Molecular Pathway Annotations     Click to see Annotation Detail View
References

References - curated
# Reference Title Reference Citation
1. Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Gaudet P, etal., Brief Bioinform. 2011 Sep;12(5):449-62. doi: 10.1093/bib/bbr042. Epub 2011 Aug 27.
2. Rat ISS GO annotations from GOA human gene data--August 2006 GOA data from the GO Consortium
3. Mutation burden analysis of six common mental disorders in African Americans by whole genome sequencing. Liu Y, etal., Hum Mol Genet. 2022 Nov 10;31(22):3769-3776. doi: 10.1093/hmg/ddac129.
4. KEGG Annotation Import Pipeline Pipeline to import KEGG annotations from KEGG into RGD
5. ClinVar Automated Import and Annotation Pipeline RGD automated import pipeline for ClinVar variants, variant-to-disease annotations and gene-to-disease annotations
6. Data Import for Chemical-Gene Interactions RGD automated import pipeline for gene-chemical interactions
Additional References at PubMed
PMID:18279312   PMID:20600662  


Genomics

Comparative Map Data
Ust
(Rattus norvegicus - Norway rat)
Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCr814,487,316 - 4,782,579 (-)NCBIGRCr8GRCr8GRCr8
GRCr8 Ensembl14,489,925 - 4,782,356 (-)EnsemblGRCr8
mRatBN7.212,666,983 - 2,962,276 (-)NCBImRatBN7.2mRatBN7.2
mRatBN7.2 Ensembl12,669,592 - 2,962,044 (-)EnsemblmRatBN7.2
UTH_Rnor_SHR_Utx12,325,889 - 2,623,315 (-)NCBIUTH_Rnor_SHR_UtxUTH_Rnor_SHR_Utx
UTH_Rnor_SHRSP_BbbUtx_1.018,326,290 - 8,623,726 (-)NCBIUTH_Rnor_SHRSP_BbbUtx_1.0UTH_Rnor_SHRSP_BbbUtx_1.0
UTH_Rnor_WKY_Bbb_1.012,608,001 - 2,905,423 (-)NCBIUTH_Rnor_WKY_Bbb_1.0UTH_Rnor_WKY_Bbb_1.0
Rnor_6.012,330,631 - 2,627,654 (-)NCBIRnor_6.0Rnor_6.0rn6Rnor6.0
Rnor_6.0 Ensembl12,333,778 - 2,627,747 (-)Ensemblrn6Rnor6.0
Rnor_5.014,022,062 - 4,313,796 (-)NCBIRnor_5.0Rnor_5.0rn5
RGSC_v3.412,868,196 - 3,158,231 (-)NCBIRGSC_v3.4RGSC_v3.4rn4
Celera11,217,279 - 1,507,424 (-)NCBICelera
RGSC_v3.112,868,195 - 3,158,231 (-)NCBI
Cytogenetic Map1p13NCBI
UST
(Homo sapiens - human)
Human AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCh386148,747,030 - 149,076,990 (+)NCBIGRCh38GRCh38hg38GRCh38
GRCh38.p14 Ensembl6148,746,913 - 149,076,991 (+)Ensemblhg38GRCh38
GRCh376149,068,166 - 149,398,126 (+)NCBIGRCh37GRCh37hg19GRCh37
Build 366149,110,157 - 149,439,818 (+)NCBIBuild 36Build 36hg18NCBI36
Build 346149,110,156 - 149,439,818NCBI
Celera6149,804,170 - 150,133,178 (+)NCBICelera
Cytogenetic Map6q25.1NCBI
HuRef6146,637,095 - 146,966,117 (+)NCBIHuRef
CHM1_16149,333,029 - 149,662,873 (+)NCBICHM1_1
T2T-CHM13v2.06149,946,826 - 150,276,839 (+)NCBIT2T-CHM13v2.0
Ust
(Mus musculus - house mouse)
Mouse AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
GRCm39108,068,499 - 8,394,701 (-)NCBIGRCm39GRCm39mm39
GRCm39 Ensembl108,080,520 - 8,394,589 (-)EnsemblGRCm39 EnsemblGRCm39
GRCm38108,204,753 - 8,518,825 (-)NCBIGRCm38GRCm38mm10GRCm38
GRCm38.p6 Ensembl108,204,756 - 8,518,825 (-)Ensemblmm10GRCm38
MGSCv37107,924,551 - 8,238,623 (-)NCBIMGSCv37MGSCv37mm9NCBIm37
MGSCv36107,897,457 - 8,208,962 (-)NCBIMGSCv36mm8
Celera108,087,930 - 8,411,124 (-)NCBICelera
Cytogenetic Map10A1NCBI
cM Map102.65NCBI
Ust
(Chinchilla lanigera - long-tailed chinchilla)
Chinchilla AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
ChiLan1.0 EnsemblNW_00495543912,125,591 - 12,406,271 (-)Ensembl
ChiLan1.0NW_00495543912,122,935 - 12,406,368 (-)NCBIChiLan1.0ChiLan1.0
UST
(Pan paniscus - bonobo/pygmy chimpanzee)
Bonobo AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
NHGRI_mPanPan1-v25168,769,032 - 169,101,939 (+)NCBINHGRI_mPanPan1-v2
NHGRI_mPanPan16166,662,491 - 166,995,609 (+)NCBINHGRI_mPanPan1
Mhudiblu_PPA_v06146,562,541 - 146,895,450 (+)NCBIMhudiblu_PPA_v0Mhudiblu_PPA_v0panPan3
PanPan1.16151,270,358 - 151,600,651 (+)NCBIPanPan1.1PanPan1.1panPan2
PanPan1.1 Ensembl6151,270,505 - 151,597,779 (+)EnsemblpanPan2panpan1.1
UST
(Canis lupus familiaris - dog)
Dog AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
CanFam3.1139,633,276 - 39,922,361 (+)NCBICanFam3.1CanFam3.1canFam3CanFam3.1
CanFam3.1 Ensembl139,633,501 - 39,921,289 (+)EnsemblcanFam3CanFam3.1
Dog10K_Boxer_Tasha140,532,475 - 40,759,827 (+)NCBIDog10K_Boxer_TashaDog10K_Boxer_Tasha
ROS_Cfam_1.0139,810,126 - 40,102,153 (+)NCBIROS_Cfam_1.0ROS_Cfam_1.0
ROS_Cfam_1.0 Ensembl139,810,043 - 40,102,150 (+)EnsemblROS_Cfam_1.0 Ensembl
UMICH_Zoey_3.1139,687,116 - 39,978,672 (+)NCBIUMICH_Zoey_3.1
UNSW_CanFamBas_1.0139,553,715 - 39,843,184 (+)NCBIUNSW_CanFamBas_1.0
UU_Cfam_GSD_1.0139,893,472 - 40,184,349 (+)NCBIUU_Cfam_GSD_1.0UU_Cfam_GSD_1.0
Ust
(Ictidomys tridecemlineatus - thirteen-lined ground squirrel)
Squirrel AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
HiC_Itri_2NW_024404946134,614,740 - 134,881,624 (+)NCBIHiC_Itri_2
SpeTri2.0 EnsemblNW_0049364891,867,249 - 2,134,874 (+)EnsemblSpeTri2.0 Ensembl
SpeTri2.0NW_0049364891,866,792 - 2,133,558 (+)NCBISpeTri2.0SpeTri2.0SpeTri2.0
UST
(Sus scrofa - pig)
Pig AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
Sscrofa11.1 Ensembl116,840,533 - 17,138,392 (-)EnsemblsusScr11Sscrofa11.1
Sscrofa11.1116,840,422 - 17,138,088 (-)NCBISscrofa11.1Sscrofa11.1susScr11Sscrofa11.1
Sscrofa10.2119,294,997 - 19,463,194 (-)NCBISscrofa10.2Sscrofa10.2susScr3
UST
(Chlorocebus sabaeus - green monkey)
Green Monkey AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
ChlSab1.11322,595,088 - 22,934,184 (-)NCBIChlSab1.1ChlSab1.1chlSab2
Vero_WHO_p1.0NW_023666040198,605,207 - 198,921,153 (-)NCBIVero_WHO_p1.0Vero_WHO_p1.0
Ust
(Heterocephalus glaber - naked mole-rat)
Naked Mole-Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
HetGla_female_1.0 EnsemblNW_0046247859,064,270 - 9,388,407 (-)EnsemblHetGla_female_1.0 EnsemblhetGla2HetGla_female_1.0 Ensembl
HetGla 1.0NW_0046247859,061,753 - 9,388,794 (-)NCBIHetGla 1.0HetGla 1.0hetGla2
Ust
(Rattus rattus - black rat)
Black Rat AssemblyChrPosition (strand)SourceGenome Browsers
JBrowseNCBIUCSCEnsembl
Rrattus_CSIRO_v12228,554,571 - 228,851,496 (+)NCBIRrattus_CSIRO_v1

Variants

.
Variants in Ust
2489 total Variants
miRNA Target Status (No longer updated)

Predicted Target Of
Summary Value
Count of predictions:32
Count of miRNA genes:32
Interacting mature miRNAs:32
Transcripts:ENSRNOT00000022003
Prediction methods:Miranda, Rnahybrid, Targetscan
Result types:miRGate_prediction

The detailed report is available here: Full Report CSV TAB Printer

miRNA Target Status data imported from miRGate (http://mirgate.bioinfo.cnio.es/).
For more information about miRGate, see PMID:25858286 or access the full paper here.


QTLs in Region (GRCr8)
The following QTLs overlap with this region.    Full Report CSV TAB Printer Gviewer
RGD IDSymbolNameLODP ValueTraitSub TraitChrStartStopSpecies
1600360Mcs16Mammary carcinoma susceptibility QTL 162.4mammary gland integrity trait (VT:0010552)mammary tumor number (CMO:0000343)1126564886Rat
70179Xhs2X-ray hypersensitivity QTL 23.2intestine integrity trait (VT:0010554)post-insult time to onset of moribundity (CMO:0001896)1143965634Rat
1354647Despr8Despair related QTL 80.0000341locomotor behavior trait (VT:0001392)amount of experiment time spent in a discrete space in an experimental apparatus (CMO:0000958)1126564886Rat
2317755Glom22Glomerulus QTL 223.8urine protein amount (VT:0005160)urine protein level (CMO:0000591)1134184556Rat
1300170Rf6Renal function QTL 63.14renal blood flow trait (VT:2000006)absolute change in renal blood flow rate (CMO:0001168)1128483164Rat

Markers in Region
RH143086  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.212,872,256 - 2,872,404 (+)MAPPERmRatBN7.2
Rnor_6.012,537,591 - 2,537,738NCBIRnor6.0
Rnor_5.014,224,374 - 4,224,521UniSTSRnor5.0
RGSC_v3.413,068,536 - 3,068,683UniSTSRGSC3.4
Celera11,418,549 - 1,418,696UniSTS
RH 3.4 Map131.9UniSTS
Cytogenetic Map1p13UniSTS
RH144477  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.212,674,912 - 2,675,113 (+)MAPPERmRatBN7.2
Rnor_6.012,339,144 - 2,339,344NCBIRnor6.0
Rnor_5.014,027,383 - 4,027,583UniSTSRnor5.0
RGSC_v3.412,873,521 - 2,873,721UniSTSRGSC3.4
Celera11,222,600 - 1,222,800UniSTS
RH 3.4 Map131.7UniSTS
Cytogenetic Map1p13UniSTS
BF389167  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.212,943,309 - 2,943,449 (+)MAPPERmRatBN7.2
mRatBN7.212,943,309 - 2,943,539 (+)MAPPERmRatBN7.2
Rnor_6.012,608,610 - 2,608,886NCBIRnor6.0
Rnor_6.012,608,610 - 2,608,749NCBIRnor6.0
Rnor_5.014,294,931 - 4,295,070UniSTSRnor5.0
Rnor_5.014,294,931 - 4,295,207UniSTSRnor5.0
RGSC_v3.413,139,503 - 3,139,642UniSTSRGSC3.4
Celera11,488,699 - 1,488,838UniSTS
RH 3.4 Map116.7UniSTS
Cytogenetic Map1p13UniSTS
RH138104  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.212,872,262 - 2,872,417 (+)MAPPERmRatBN7.2
Rnor_6.012,537,597 - 2,537,751NCBIRnor6.0
Rnor_5.014,224,380 - 4,224,534UniSTSRnor5.0
RGSC_v3.413,068,542 - 3,068,696UniSTSRGSC3.4
Celera11,418,555 - 1,418,709UniSTS
Cytogenetic Map1p13UniSTS
RH138858  
Rat AssemblyChrPosition (strand)SourceJBrowse
GRCr814,506,146 - 4,506,348 (+)Marker Load Pipeline
mRatBN7.212,685,816 - 2,686,018 (+)MAPPERmRatBN7.2
Rnor_6.012,350,045 - 2,350,246NCBIRnor6.0
Rnor_5.014,038,284 - 4,038,485UniSTSRnor5.0
RGSC_v3.412,884,422 - 2,884,623UniSTSRGSC3.4
Celera11,233,501 - 1,233,702UniSTS
RH 3.4 Map116.7UniSTS
Cytogenetic Map1p13UniSTS
BI276377  
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.212,931,413 - 2,931,569 (+)MAPPERmRatBN7.2
Rnor_6.012,596,740 - 2,596,895NCBIRnor6.0
Rnor_5.014,283,280 - 4,283,435UniSTSRnor5.0
RGSC_v3.413,127,742 - 3,127,897UniSTSRGSC3.4
Celera11,477,089 - 1,477,244UniSTS
RH 3.4 Map116.7UniSTS
Cytogenetic Map1p13UniSTS


Expression

RNA-SEQ Expression

alimentary part of gastrointestinal system
appendage
circulatory system
ectoderm
endocrine system
endoderm
exocrine system
hemolymphoid system
hepatobiliary system
integumental system
mesenchyme
mesoderm
musculoskeletal system
nervous system
renal system
reproductive system
respiratory system
sensory system
visual system
19 13 71 196 90 89 58 147 58 6 437 254 14 181 98 108 31 26 26

Sequence


Ensembl Acc Id: ENSRNOT00000022003   ⟹   ENSRNOP00000022003
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr8 Ensembl14,489,925 - 4,782,356 (-)Ensembl
mRatBN7.2 Ensembl12,669,592 - 2,962,044 (-)Ensembl
Rnor_6.0 Ensembl12,333,778 - 2,627,747 (-)Ensembl
Ensembl Acc Id: ENSRNOT00000154161   ⟹   ENSRNOP00000103555
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr8 Ensembl14,489,925 - 4,782,129 (-)Ensembl
Ensembl Acc Id: ENSRNOT00000171018   ⟹   ENSRNOP00000111073
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr8 Ensembl14,489,925 - 4,782,356 (-)Ensembl
RefSeq Acc Id: NM_001108458   ⟹   NP_001101928
RefSeq Status: PROVISIONAL
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr814,489,925 - 4,782,356 (-)NCBI
mRatBN7.212,669,592 - 2,962,044 (-)NCBI
Rnor_6.012,333,823 - 2,627,475 (-)NCBI
Rnor_5.014,022,062 - 4,313,796 (-)NCBI
RGSC_v3.412,868,196 - 3,158,231 (-)RGD
Celera11,217,279 - 1,507,424 (-)RGD
Sequence:
RefSeq Acc Id: XM_039081058   ⟹   XP_038936986
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr814,492,128 - 4,782,579 (-)NCBI
mRatBN7.212,674,894 - 2,962,276 (-)NCBI
RefSeq Acc Id: XM_039081060   ⟹   XP_038936988
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr814,487,316 - 4,658,968 (-)NCBI
mRatBN7.212,666,983 - 2,826,011 (-)NCBI
RefSeq Acc Id: XM_063265960   ⟹   XP_063122030
Type: CODING
Position:
Rat AssemblyChrPosition (strand)Source
GRCr814,487,316 - 4,782,569 (-)NCBI
RefSeq Acc Id: NP_001101928   ⟸   NM_001108458
- UniProtKB: D3ZPB6 (UniProtKB/TrEMBL),   A6JP23 (UniProtKB/TrEMBL)
- Sequence:
Ensembl Acc Id: ENSRNOP00000022003   ⟸   ENSRNOT00000022003
RefSeq Acc Id: XP_038936988   ⟸   XM_039081060
- Peptide Label: isoform X3
RefSeq Acc Id: XP_038936986   ⟸   XM_039081058
- Peptide Label: isoform X2
RefSeq Acc Id: XP_063122030   ⟸   XM_063265960
- Peptide Label: isoform X1
Ensembl Acc Id: ENSRNOP00000103555   ⟸   ENSRNOT00000154161
Ensembl Acc Id: ENSRNOP00000111073   ⟸   ENSRNOT00000171018

Protein Structures
Name Modeler Protein Id AA Range Protein Structure
AF-D3ZPB6-F1-model_v2 AlphaFold D3ZPB6 1-408 view protein structure

Transcriptome

eQTL   View at Phenogen
WGCNA   View at Phenogen
Tissue/Strain Expression   View at Phenogen

Promoters
RGD ID:13689483
Promoter ID:EPDNEW_R8
Type:multiple initiation site
Name:Ust_1
Description:uronyl-2-sulfotransferase
SO ACC ID:SO:0000170
Source:EPDNEW (Eukaryotic Promoter Database, http://epd.vital-it.ch/)
Experiment Methods:Single-end sequencing.
Position:
Rat AssemblyChrPosition (strand)Source
Rnor_6.012,627,523 - 2,627,583EPDNEW

Additional Information

Database Acc Id Source(s)
AGR Gene RGD:1304743 AgrOrtholog
BioCyc Gene G2FUF-62439 BioCyc
Ensembl Genes ENSRNOG00000016381 Ensembl, ENTREZGENE, UniProtKB/TrEMBL
Ensembl Transcript ENSRNOT00000022003 ENTREZGENE
  ENSRNOT00000022003.7 UniProtKB/TrEMBL
  ENSRNOT00000154161 ENTREZGENE
  ENSRNOT00000154161.1 UniProtKB/TrEMBL
  ENSRNOT00000171018.1 UniProtKB/TrEMBL
Gene3D-CATH 3.40.50.300 UniProtKB/TrEMBL
InterPro Heparan_SO4_2-O-STrfase UniProtKB/TrEMBL
  P-loop_NTPase UniProtKB/TrEMBL
  Sulfotransferase UniProtKB/TrEMBL
KEGG Report rno:361450 UniProtKB/TrEMBL
NCBI Gene 361450 ENTREZGENE
PANTHER HS2ST UniProtKB/TrEMBL
  URONYL 2-SULFOTRANSFERASE UniProtKB/TrEMBL
Pfam Sulfotransfer_2 UniProtKB/TrEMBL
PhenoGen Ust PhenoGen
RatGTEx ENSRNOG00000016381 RatGTEx
Superfamily-SCOP SSF52540 UniProtKB/TrEMBL
UniProt A0ABK0LDS5_RAT UniProtKB/TrEMBL
  A0ABK0M8R7_RAT UniProtKB/TrEMBL
  A6JP23 ENTREZGENE, UniProtKB/TrEMBL
  D3ZPB6 ENTREZGENE, UniProtKB/TrEMBL


Nomenclature History
Date Current Symbol Current Name Previous Symbol Previous Name Description Reference Status
2008-04-30 Ust  uronyl-2-sulfotransferase   Ust_predicted  uronyl-2-sulfotransferase (predicted)  'predicted' is removed 2292626 APPROVED
2005-01-12 Ust_predicted  uronyl-2-sulfotransferase (predicted)      Symbol and Name status set to approved 70820 APPROVED