RGD Reference Report - The role of genetic markers--NAP1L1, MAGE-D2, and MTA1--in defining small-intestinal carcinoid neoplasia. - Rat Genome Database

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The role of genetic markers--NAP1L1, MAGE-D2, and MTA1--in defining small-intestinal carcinoid neoplasia.

Authors: Kidd, M  Modlin, IM  Mane, SM  Camp, RL  Eick, G  Latich, I 
Citation: Kidd M, etal., Ann Surg Oncol. 2006 Feb;13(2):253-62. Epub 2006 Jan 20.
RGD ID: 9590082
Pubmed: PMID:16424981   (View Abstract at PubMed)
DOI: DOI:10.1245/ASO.2006.12.011   (Journal Full-text)

BACKGROUND: Standard clinical and immunohistochemical methods cannot reliably determine whether a small intestinal carcinoid (SIC) is indolent or aggressive. We hypothesized that carcinoid malignancy could be defined by using quantitative reverse transcriptase-polymerase chain reaction (QRT-PCR) and immunohistochemical approaches that evaluate potential marker genes. METHODS: Candidate marker gene expression (nucleosome assembly protein 1-like 1 [NAP1L1], melanoma antigen D2 [MAGE-D2], and metastasis-associated protein 1 [MTA1]) identified by Affymetrix transcriptional profiling was examined by QRT-PCR in SIC, liver, and lymph node (LN) metastases, colorectal carcinomas, and healthy tissues. Immunohistochemical expression levels of MTA1 were analyzed quantitatively by a novel automated quantitative analysis in a tissue microarray of 102 gastrointestinal carcinoids and in a breast/prostate carcinoma array. RESULTS: Affymetrix transcriptional profiling identified three potentially useful malignancy-marker genes (out of 1709 significantly altered genes). By QRT-PCR, NAP1L1 was significantly (P < .03) overexpressed in SIC compared with colorectal carcinomas and healthy tissue. Increased levels (P < .05) were identified in both liver and LN metastases. Levels in colorectal carcinomas were the same as in healthy mucosa. MAGE-D2 and MTA1 were increased (P < .05) in primary tumors and metastases and overexpressed in carcinomas. Automated quantitative analysis demonstrated the highest levels of MTA1 immunostaining in malignant primary SICs and in metastases to the liver and LN. These were significantly increased (P < .02) compared with nonmetastatic primary tumors. MTA1 was overexpressed in breast and prostate carcinomas (P < .05). CONCLUSIONS: SICs overexpress the neoplasia-related genes NAP1L1 (mitotic regulation), MAGE-D2 (adhesion), and MTA1 (estrogen antagonism). The ability to determine the malignant potential of these tumors and their propensity to metastasize provides a biological rationale for the management of carcinoids and may have prognostic utility.



RGD Manual Disease Annotations    Click to see Annotation Detail View
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NAP1L1HumanIntestinal Carcinoid Tumors  IEP mRNA:increased expression:intestinal mucosa (human)RGD 
Nap1l1RatIntestinal Carcinoid Tumors  ISONAP1L1 (Homo sapiens)mRNA:increased expression:intestinal mucosa (human)RGD 
Nap1l1MouseIntestinal Carcinoid Tumors  ISONAP1L1 (Homo sapiens)mRNA:increased expression:intestinal mucosa (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nap1l1  (nucleosome assembly protein 1-like 1)

Genes (Mus musculus)
Nap1l1  (nucleosome assembly protein 1-like 1)

Genes (Homo sapiens)
NAP1L1  (nucleosome assembly protein 1 like 1)


Additional Information