RGD Reference Report - Disturbing miR-182 and -381 inhibits BRD7 transcription and glioma growth by directly targeting LRRC4. - Rat Genome Database

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Disturbing miR-182 and -381 inhibits BRD7 transcription and glioma growth by directly targeting LRRC4.

Authors: Tang, H  Wang, Z  Liu, Q  Liu, X  Wu, M  Li, G 
Citation: Tang H, etal., PLoS One. 2014 Jan 3;9(1):e84146. doi: 10.1371/journal.pone.0084146. eCollection 2014.
RGD ID: 9586441
Pubmed: PMID:24404152   (View Abstract at PubMed)
PMCID: PMC3880275   (View Article at PubMed Central)
DOI: DOI:10.1371/journal.pone.0084146   (Journal Full-text)

Inactivated LRRC4 has been clinically detected in gliomas, and promoter hypermethylation has been implicated as the mechanism of inactivation in some of those tumors. Our previous researches indicated that LRRC4 is a target gene of miR-381, the interaction of miR-381 and LRRC4 is involved in glioma growth. In this study, we demonstrate that LRRC4 is a target gene of the other microRNA, miR-182. We found that the high expression of miR-182 and miR-381 in gliomas are involved in pathological malignant progression. The silencing of miR-182 and miR-381 inhibited the proliferation in vitro and growth of glioma cell with in vivo magnetic resonance imaging by intracranial transplanted tumor model in rats. We also demonstrated that BRD7, a transcriptional cofactor for p53, is highly expressed and negatively correlated with LRRC4 expression in gliomas. Disturbing miR-182 and miR-381 affected transcriptional regulation of the BRD7 gene. This finding was verified by ectopic overexpression of LRRC4 or restoration of endogenous LRRC4 expression by treatment with the DNA demethylating agent 5-Aza-dC. Taken together, miR-182 and miR-381 may be a useful therapeutic target for treatment of glioma.



RGD Manual Disease Annotations    Click to see Annotation Detail View
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
BRD7Humanglioblastoma  IEP protein:increased expression:brain:RGD 
Brd7Ratglioblastoma  ISOBRD7 (Homo sapiens)protein:increased expression:brain:RGD 
Brd7Mouseglioblastoma  ISOBRD7 (Homo sapiens)protein:increased expression:brain:RGD 
BRD7Humanhigh grade glioma  IEP protein:increased expression:brain:RGD 
Brd7Rathigh grade glioma  ISOBRD7 (Homo sapiens)protein:increased expression:brain:RGD 
Brd7Mousehigh grade glioma  ISOBRD7 (Homo sapiens)protein:increased expression:brain:RGD 
BRD7Humanmalignant astrocytoma severityIEP protein:increased expression:brain:RGD 
Brd7Ratmalignant astrocytoma severityISOBRD7 (Homo sapiens)protein:increased expression:brain:RGD 
Brd7Mousemalignant astrocytoma severityISOBRD7 (Homo sapiens)protein:increased expression:brain:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Brd7  (bromodomain containing 7)

Genes (Mus musculus)
Brd7  (bromodomain containing 7)

Genes (Homo sapiens)
BRD7  (bromodomain containing 7)


Additional Information