RGD Reference Report - Cloning and expression of a bovine glutamate transporter. - Rat Genome Database

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Cloning and expression of a bovine glutamate transporter.

Authors: Inoue, K  Sakaitani, M  Shimada, S  Tohyama, M 
Citation: Inoue K, etal., Brain Res Mol Brain Res. 1995 Feb;28(2):343-8.
RGD ID: 8553776
Pubmed: PMID:7723632   (View Abstract at PubMed)

We have isolated a 3845 base-pair cDNA (BNGLUAS) encoding a bovine glutamate transporter (bovine GLAST) by screening a bovine retina cDNA library with an oligonucleotide probe corresponding to conserved regions of known glutamate transporters. The cDNA sequence predicted a protein of 542 amino acids and displayed 96% and 97% amino acid identity with the rat GLAST/GluT-1 and human GLAST, respectively. Expression of the bovine GLAST in Xenopus oocytes revealed Na(+)-dependent [14C]L-glutamate uptake and electrogenic glutamate uptake.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc1a3RatL-glutamate transmembrane transport acts_upstream_of_or_withinIDA PMID:7723632MGI 
Slc1a3Ratresponse to inorganic substance acts_upstream_of_or_withinIDA PMID:7723632MGI 

Objects Annotated

Genes (Rattus norvegicus)
Slc1a3  (solute carrier family 1 member 3)


Additional Information