RGD Reference Report - Molecular cloning and functional expression of a fifth-type alpha 2,3-sialyltransferase (mST3Gal V: GM3 synthase). - Rat Genome Database

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Molecular cloning and functional expression of a fifth-type alpha 2,3-sialyltransferase (mST3Gal V: GM3 synthase).

Authors: Kono, M  Takashima, S  Liu, H  Inoue, M  Kojima, N  Lee, YC  Hamamoto, T  Tsuji, S 
Citation: Kono M, etal., Biochem Biophys Res Commun 1998 Dec 9;253(1):170-5.
RGD ID: 724694
Pubmed: PMID:9875239   (View Abstract at PubMed)

The cDNA encoding a new type of alpha 2,3-sialyltransferase (mST3Gal V) was cloned from mouse brain cDNA library by PCR-based cloning approach using a pair of degenerate primers deduced from the nucleotide sequence information of mouse ST3Gal III and IV. The predicted amino acid sequence of mST3Gal V showed 27.3% and 26.4% identity to mST3Gal III and IV, respectively. The recombinant soluble mST3Gal V fused with protein-A, which expressed in the culture media of COS-7 cells, showed activity toward lactosylceramide (LacCer), and synthesized GM3. The apparent Km value for LacCer was 9.3 microM. mST3Gal V did not exhibit any activity toward other substrates we tested in this study, including glycolipids, glycoproteins and disaccharides. The mST3Gal V cDNA transfected F28-7 cells, which express large amount of lactosylceramide and very small amount of GM3 at native stage, expressed a large amount of GM3. The ST3Gal V gene was strongly expressed in mouse brain and liver, which contained a large amount of ganglioside. The gene expression seemed to be coincident with ganglioside expression in mouse. Thus, we conclude that mST3Gal V is the fifth-type alpha 2,3-sialyltransferase carrying GM3 synthetic activity.



Objects referenced in this article
Gene St3gal5 ST3 beta-galactoside alpha-2,3-sialyltransferase 5 Mus musculus
Gene St3gal5 ST3 beta-galactoside alpha-2,3-sialyltransferase 5 Rattus norvegicus
Gene Usf2 upstream transcription factor 2, c-fos interacting Rattus norvegicus

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