RGD Reference Report - Cloning of the rat heart Na(+)-Ca2+ exchanger and its functional expression in HeLa cells. - Rat Genome Database

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Cloning of the rat heart Na(+)-Ca2+ exchanger and its functional expression in HeLa cells.

Authors: Low, W  Kasir, J  Rahamimoff, H 
Citation: Low W, etal., FEBS Lett 1993 Jan 18;316(1):63-7.
RGD ID: 70014
Pubmed: PMID:8422940   (View Abstract at PubMed)

A functional rat heart Na(+)-Ca2+ exchanger gene has been obtained by splicing and ligating two partially overlapping clones isolated from a rat heart lambda ZAP cDNA library. The deduced primary structure of the protein encoded by the open reading frame corresponds to 971 amino acids, that can be organized into 12 transmembrane helices. The cloned gene was functionally expressed in HeLa cells. Maximal expression was detected 18 h after transfection, after which transport activity rapidly declined. The electrogenic properties of the cloned transporter were demonstrated following reconstitution of the expressed exchanger protein into a tightly sealed phospholipid membrane.

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Gene Slc8a1 solute carrier family 8 member A1 Rattus norvegicus

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