RGD Reference Report - Epidermal growth factor reduces autophagy in intestinal epithelium and in the rat model of necrotizing enterocolitis. - Rat Genome Database

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Epidermal growth factor reduces autophagy in intestinal epithelium and in the rat model of necrotizing enterocolitis.

Authors: Maynard, AA  Dvorak, K  Khailova, L  Dobrenen, H  Arganbright, KM  Halpern, MD  Kurundkar, AR  Maheshwari, A  Dvorak, B 
Citation: Maynard AA, etal., Am J Physiol Gastrointest Liver Physiol. 2010 Sep;299(3):G614-22. Epub 2010 Jun 10.
RGD ID: 6483081
Pubmed: PMID:20539009   (View Abstract at PubMed)
PMCID: PMC2950687   (View Article at PubMed Central)
DOI: DOI:10.1152/ajpgi.00076.2010   (Journal Full-text)

Necrotizing enterocolitis (NEC) is a devastating intestinal disease of premature infants. Epidermal growth factor (EGF) is one of the most promising candidates in NEC prophylaxis. Autophagy regulates cell homeostasis, but uncontrolled activation of autophagy may lead to cellular injury. The aim was to evaluate the effects of EGF on intestinal autophagy in epithelial cells and in the rat NEC model and measure autophagy in NEC patients. Intestinal epithelial cells (IEC-6) and the rat NEC model were used to study the effect of EGF on intestinal autophagy. Protein levels of Beclin 1 and LC3II were measured in the intestinal epithelium in both in vivo and in vitro models. Ultrastructural changes in intestinal epithelium were studied by electron microscopy. Expression of Beclin 1, LC3II, and p62 protein was evaluated in biopsies from NEC patients. Autophagy was induced in IEC-6 cells and inhibited by adding EGF into the culture. In the rat NEC model, EGF treatment of NEC reduced expression of Beclin 1 and LC3II in ileal epithelium. Morphologically, typical signs of autophagy were observed in the epithelium of the NEC group, but not in the EGF group. A strong signal for Beclin 1 and LC3II was detected in the intestine from patients with NEC. Autophagy is activated in the intestinal epithelium of NEC patients and in the ileum of NEC rats. Supplementation of EGF blocks intestinal autophagy in both in vivo and in vitro conditions. Results from this study indicate that EGF-mediated protection against NEC injury is associated with regulation of intestinal autophagy.



RGD Manual Disease Annotations    Click to see Annotation Detail View
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
BECN1Humanperinatal necrotizing enterocolitis  IEP protein:increased expression:intestineRGD 
Becn1Ratperinatal necrotizing enterocolitis  ISOBECN1 (Homo sapiens)protein:increased expression:intestineRGD 
Becn1Mouseperinatal necrotizing enterocolitis  ISOBECN1 (Homo sapiens)protein:increased expression:intestineRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Becn1Ratcellular response to epidermal growth factor stimulus  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Becn1  (beclin 1)

Genes (Mus musculus)
Becn1  (beclin 1, autophagy related)

Genes (Homo sapiens)
BECN1  (beclin 1)


Additional Information