RGD Reference Report - Enzymatic properties, tissue-specific expression, and lysosomal location of two highly homologous rat SULT1C2 sulfotransferases. - Rat Genome Database

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Enzymatic properties, tissue-specific expression, and lysosomal location of two highly homologous rat SULT1C2 sulfotransferases.

Authors: Xiangrong, L  Johnk, C  Hartmann, D  Schestag, F  Kromer, W  Gieselmann, V 
Citation: Xiangrong L, etal., Biochem Biophys Res Commun 2000 May 27;272(1):242-50.
RGD ID: 634073
Pubmed: PMID:10872834   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.2000.2744   (Journal Full-text)

We have isolated two highly homologous but distinct rat sulfotransferase cDNAs termed ratSULT1C2 and ratSULT1C2A encoding polypeptides of 297 amino acids each. The amino acid sequence of ratSULT1C2 is 84% identical to the human SULT1C2 and 81% identical to a rabbit SULT1C2 sulfotransferase. ratSULT1C2 and ratSULT1C2A are 92% identical but differ in 22 amino acids. The majority of these amino acid substitutions in ratSULT1C2A is not found in the human and rabbit SULT1C2, which identifies ratSULT1C2 as the orthologue of these sulfotransferases, whereas SULT1C2A is a closely related but distinct enzyme. ratSULT1C2 and 2A sulfotransferases do not sulfonate steroids, dopamine, acetaminophen, or alpha-naphthol, but only p-nitrophenol. Prokaryotically expressed ratSULT1C2A is less active than ratSULT1C2. ratSULT1C2/2A mRNAs are abundant in kidney and less abundant in stomach and liver. The enzymes are expressed as 34-kDa polypeptides in rat kidney, liver, and stomach. In addition, a 28-kDa cross-reacting polypeptide is found in kidney only. Immunohistochemistry revealed expression of ratSULT1C2/2A in the epithelial cells of the proximal tubules of the kidney, bile duct epithelia, hepatocytes, and the epithelium of the gastric mucosal glands. Although the cDNA predicted amino acid sequence identifies both sulfotransferases as cytosolic enzymes, in tissue sections, in the kidney cell line NRK 52, and in transiently transfected BHK cells a considerable fraction of the enzyme was found in a granular perinuclear compartment. Costaining with a lysosomal marker in gastric mucosa tissue sections and cultured cells identifies these structures as lysosomes.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
LOC120093086Ratsulfation involved_inIDA PMID:10872834UniProt 
Sult1c2Ratsulfation involved_inIDA PMID:10872834UniProt 
Sult1c2aRatsulfation involved_inIDA PMID:10872834UniProt 

Cellular Component
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
LOC120093086Ratlysosome located_inIDA PMID:10872834UniProt 
Sult1c2Ratlysosome located_inIDA PMID:10872834UniProt 
Sult1c2aRatlysosome located_inIDA PMID:10872834UniProt 

Molecular Function
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
LOC120093086Rataryl sulfotransferase activity enablesIDA PMID:10872834UniProt 
Sult1c2Rataryl sulfotransferase activity enablesIDA PMID:10872834UniProt 
Sult1c2aRataryl sulfotransferase activity enablesIDA PMID:10872834UniProt 

Objects Annotated

Genes (Rattus norvegicus)
LOC120093086  (sulfotransferase 1C2-like)
Sult1c2  (sulfotransferase family 1C member 2)
Sult1c2a  (sulfotransferase family 1C member 2A)


Additional Information