RGD Reference Report - cDNA cloning and characterization of Drb1, a new member of RRM-type neural RNA-binding protein. - Rat Genome Database

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cDNA cloning and characterization of Drb1, a new member of RRM-type neural RNA-binding protein.

Authors: Tamada, H  Sakashita, E  Shimazaki, K  Ueno, E  Hamamoto, T  Kagawa, Y  Endo, H 
Citation: Tamada H, etal., Biochem Biophys Res Commun 2002 Sep 13;297(1):96-104.
RGD ID: 632617
Pubmed: PMID:12220514   (View Abstract at PubMed)

Neural RNA recognition motif (RRM)-type RNA-binding proteins play essential roles in neural development. To search for a new member of neural RRM-type RNA-binding protein, we screened rat cerebral expression library with polyclonal antibody against consensus RRM sequences. We have cloned and characterized a rat cDNA that belongs to RRM-type RNA-binding protein family, which we designate as drb1. Orthologs of drb1 exist in human and mouse. The predicted amino acid sequence reveals an open reading frame of 476 residues with a corresponding molecular mass of 53kDa and consists of four RNA-binding domains. drb1 gene is specifically expressed in fetal (E12, E16) rat brain and gradually reduced during development. In situ hybridization demonstrated neuron-specific signals in fetal rat brain. RNA-binding assay indicated that human Drb1 protein possesses binding preference on poly(C)RNA. These results indicate that Drb1 is a new member of neural RNA-binding proteins, which expresses under spatiotemporal control.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Rbm45Ratnervous system development involved_inIEP PMID:12220514UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Rbm45  (RNA binding motif protein 45)


Additional Information