RGD Reference Report - Identification of a plasma membrane glutamine transporter from the rat hepatoma cell line H4-IIE-C3. - Rat Genome Database

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Identification of a plasma membrane glutamine transporter from the rat hepatoma cell line H4-IIE-C3.

Authors: Pollard, M  Meredith, D  McGivan, JD 
Citation: Pollard M, etal., Biochem J 2002 Nov 15;368(Pt 1):371-5.
RGD ID: 628314
Pubmed: PMID:12171599   (View Abstract at PubMed)
PMCID: PMC1222977   (View Article at PubMed Central)
DOI: DOI:10.1042/BJ20020982   (Journal Full-text)

Glutamine is taken up into the rat hepatoma cell line H4-IIE-C3 by a Na(+)-dependent transport system which is specific for glutamine, alanine, serine, cysteine and asparagine and does not tolerate substitution of Na(+) by Li(+). Glutamine transport was relatively weakly inhibited by a 50-fold excess of leucine and was not inhibited by phenylalanine or N -methyl aminoisobutyrate. These general properties are characteristic of the recently identified ASCT/B(0) family of transporters. Using a reverse transcriptase PCR-based homology cloning approach, we have characterized a cDNA for a novel member of this transporter family (H4-ASCT2) from H4-IIE-C3 cells. The cDNA encodes a 551-amino acid protein which exhibits similarities of between 75 and 85% with ASCT/B(0) transporters previously cloned from other sources. When expressed in Xenopus oocytes, this transporter catalyses Na(+)-dependent glutamine uptake with characteristics very similar to those of glutamine uptake into the H4-IIE-C3 cells. This newly characterized transporter possesses a number of amino acid sequence differences from ASCT2 clones recently isolated from rat astroglial cells and from normal rat liver. In particular, the loop region between transmembrane helices 3 and 4 from H4-ASCT2 shares less than 60% sequence similarity with ASCT2 from rat liver; furthermore, there are some 25 single amino acid substitutions elsewhere in the H4-ASCT2 sequence compared with that from rat liver. Thus enhanced glutamine uptake in rat hepatoma cells is mediated by the expression of a novel ASCT/B(0) transporter isoform rather than by increased expression of the ASCT2 mRNA found in normal rat liver.



Objects referenced in this article
Gene Slc1a5 solute carrier family 1 member 5 Rattus norvegicus

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