RGD Reference Report - Progranulin deficiency decreases gross neural connectivity but enhances transmission at individual synapses. - Rat Genome Database

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Progranulin deficiency decreases gross neural connectivity but enhances transmission at individual synapses.

Authors: Tapia, L  Milnerwood, A  Guo, A  Mills, F  Yoshida, E  Vasuta, C  Mackenzie, IR  Raymond, L  Cynader, M  Jia, W  Bamji, SX 
Citation: Tapia L, etal., J Neurosci. 2011 Aug 3;31(31):11126-32.
RGD ID: 5509044
Pubmed: PMID:21813674   (View Abstract at PubMed)
PMCID: PMC6623368   (View Article at PubMed Central)
DOI: DOI:10.1523/JNEUROSCI.6244-10.2011   (Journal Full-text)

Frontotemporal dementia (FTD) has been linked to mutations in the progranulin gene (GRN) that lead to progranulin (PGRN) haploinsufficiency. Thus far, our understanding of the effects of PGRN depletion in the brain has been derived from investigation of gross pathology, and more detailed analyses of cellular function have been lacking. We report that knocking down PGRN levels in rat primary hippocampal cultures reduces neural connectivity by decreasing neuronal arborization and length as well as synapse density. Despite this, the number of synaptic vesicles per synapse and the frequency of mEPSCs are increased in PGRN knockdown cells, suggesting an increase in the probability of release at remaining synapses. Interestingly, we demonstrate that the number of vesicles per synapse is also increased in postmortem brain sections from FTD patients with PGRN haploinsufficiency, relative to controls. Our observations show that PGRN knockdown severely alters neuronal connectivity in vitro and that the synaptic vesicle phenotype observed in culture is consistent with that observed in the hippocampus of FTD patients.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
GrnRatpositive regulation of dendritic spine development  IMP  RGD 
GrnRatpositive regulation of neuron projection development  IMP  RGD 
GrnRatsynaptic vesicle endocytosis  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Grn  (granulin precursor)


Additional Information