RGD Reference Report - Mammalian monocarboxylate transporter 7 (MCT7/Slc16a6) is a novel facilitative taurine transporter. - Rat Genome Database

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Mammalian monocarboxylate transporter 7 (MCT7/Slc16a6) is a novel facilitative taurine transporter.

Authors: Higuchi, Kei  Sugiyama, Koki  Tomabechi, Ryuto  Kishimoto, Hisanao  Inoue, Katsuhisa 
Citation: Higuchi K, etal., J Biol Chem. 2022 Apr;298(4):101800. doi: 10.1016/j.jbc.2022.101800. Epub 2022 Mar 5.
RGD ID: 329955553
Pubmed: PMID:35257743   (View Abstract at PubMed)
PMCID: PMC8980330   (View Article at PubMed Central)
DOI: DOI:10.1016/j.jbc.2022.101800   (Journal Full-text)

Monocarboxylate transporter 7 (MCT7) is an orphan transporter expressed in the liver, brain, and in several types of cancer cells. It has also been reported to be a survival factor in melanoma and breast cancers. However, this survival mechanism is not yet fully understood due to MCT7's unidentified substrate(s). Therefore, here we sought to identify MCT7 substrate(s) and characterize the transport mechanisms by analyzing amino acid transport in HEK293T cells and polarized Caco-2 cells. Analysis of amino acids revealed significant rapid reduction in taurine from cells transfected with enhanced green fluorescent protein-tagged MCT7. We found that taurine uptake and efflux by MCT7 was pH-independent and that the uptake was not saturated in the presence of taurine excess of 200 mM. Furthermore, we found that monocarboxylates and acidic amino acids inhibited MCT7-mediated taurine uptake. These results imply that MCT7 may be a low-affinity facilitative taurine transporter. We also found that MCT7 was localized at the basolateral membrane in polarized Caco-2 cells and that the induction of MCT7 expression in polarized Caco-2 cells enhanced taurine permeation. Finally, we demonstrated that interactions of MCT7 with ancillary proteins basigin/CD147 and embigin/GP70 enhanced MCT7-mediated taurine transport. In summary, these findings reveal that taurine is a novel substrate of MCT7 and that MCT7-mediated taurine transport might contribute to the efflux of taurine from cells.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc16a6Ratbasolateral plasma membrane located_inIDA PMID:35257743UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Slc16a6  (solute carrier family 16, member 6)


Additional Information