RGD Reference Report - Neuroprotective properties of ciliary neurotrophic factor for cultured adult rat dorsal root ganglion neurons. - Rat Genome Database

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Neuroprotective properties of ciliary neurotrophic factor for cultured adult rat dorsal root ganglion neurons.

Authors: Sango, K  Yanagisawa, H  Komuta, Y  Si, Y  Kawano, H 
Citation: Sango K, etal., Histochem Cell Biol. 2008 Oct;130(4):669-79. Epub 2008 Aug 5.
RGD ID: 2326061
Pubmed: PMID:18679704   (View Abstract at PubMed)
DOI: DOI:10.1007/s00418-008-0484-x   (Journal Full-text)

We observed that recombinant ciliary neurotrophic factor (CNTF) enhanced survival and neurite outgrowth of cultured adult rat dorsal root ganglion (DRG) neurons. Among other neurotrophic factors (NGF and GDNF) and interleukin (IL)-6 cytokine members [IL-6, LIF, cardiotrophin-1, and oncostatin M (OSM)] at the same concentration (50 ng/ml), CNTF, as well as LIF and OSM, displayed high efficacy for the promotion of the number of viable neurons and neurite-bearing cells. CNTF enhanced the number of neurite-bearing cells in both small neurons (soma diameter <30 microm) and large neurons (soma diameter > or =30 microm), whereas NGF and GDNF promoted that in only small neurons. Western blot analysis revealed that CNTF induced phosphorylation of STAT3, Akt, and ERK1/2 in the neurons. Furthermore, the neurite outgrowth-promoting activity of CNTF was diminished by co-treatment with Janus kinase (JAK) 2 inhibitor, AG490; STAT3 inhibitor, STA-21; phosphatidyl inositol-3'-phosphate-kinase (PI3K) inhibitor, LY294002; and mitogen-activated protein kinase kinase (MEK) inhibitor, PD98059, in a concentration-dependent manner. Its survival-promoting activity was also affected by AG490, STA-21, and LY294002 at higher concentrations, but not by PD98059. These findings suggest the involvement of JAK2/STAT3, PI3K/Akt, and MEK/ERK signaling pathways in CNTF-induced neurite outgrowth, where the former two pathways are thought to play major roles in mediating the survival response of neurons to CNTF.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
LifRatpositive regulation of neuron projection development  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Lif  (LIF, interleukin 6 family cytokine)


Additional Information