RGD Reference Report - Up-regulation of MUC5AC mRNA expression in endotoxin-induced otitis media. - Rat Genome Database

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Up-regulation of MUC5AC mRNA expression in endotoxin-induced otitis media.

Authors: Kim, YT  Jung, HH  Ko, TO  Kim, SJ 
Citation: Kim YT, etal., Acta Otolaryngol. 2001 Apr;121(3):364-70.
RGD ID: 2324973
Pubmed: PMID:11425202   (View Abstract at PubMed)

We investigated the expression levels of MUC5AC in endotoxin-induced otitis media with effusion (OME) in the rat using competitive polymerase chain reaction (PCR) and the morphology of middle ear mucosa using transmission electron microscopy (TEM). Experimental OME in the rat was induced after middle ear instillation of Escherichia coli lipopolysaccharides (LPS). Middle ear mucosa were obtained at 0 h, 12 h, Day 1, Day 3, Day 7 and Day 14 and reverse transcriptase (RT)-PCRs were then performed for the identification of MUC1, MUC2, MUC5AC and submandibular mucin 1 expression, followed by competitive PCRs for MUC5AC and beta2-microglobulin expression. Normal middle ear mucosa revealed no expression of mucin genes, whereas endotoxin upregulated the expression of MUC5AC mRNA between 12 h and Day 7, with maximal expression at Days 1 and 3. Middle ears treated three times with LPS upregulated more MUC5AC mRNA expression, by a factor of approximately 3.5, than those 1 day after one instillation. On TEM, dark granulated cells were observed at Day 3 after endotoxin instillation, but mixed granulated cells were seen on the ears treated three times with LPS. These results suggest that MUC5AC could be one of the major mucin genes in the middle ear mucosa related to otitis media.



RGD Manual Disease Annotations    Click to see Annotation Detail View
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MUC5ACHumanotitis media  ISOMuc5ac (Rattus norvegicus)mRNA:increased expression:earRGD 
Muc5acRatotitis media  IEP mRNA:increased expression:earRGD 
Muc5acMouseotitis media  ISOMuc5ac (Rattus norvegicus)mRNA:increased expression:earRGD 

Objects Annotated

Genes (Rattus norvegicus)
Muc5ac  (mucin 5AC, oligomeric mucus/gel-forming)

Genes (Mus musculus)
Muc5ac  (mucin 5, subtypes A and C, tracheobronchial/gastric)

Genes (Homo sapiens)
MUC5AC  (mucin 5AC, oligomeric mucus/gel-forming)


Additional Information