RGD Reference Report - Biosynthesis of pyruvate kinase isozymes in rat liver. - Rat Genome Database

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Biosynthesis of pyruvate kinase isozymes in rat liver.

Authors: Wu, SW  Wong, SS  Yeung, DC 
Citation: Wu SW, etal., Eur J Biochem. 1981 Dec;121(1):59-63.
RGD ID: 2303137
Pubmed: PMID:7327170   (View Abstract at PubMed)

1. The L and M1 isozymes of pyruvate kinase were purified to homogeneity from rat liver and muscle respectively and their specific antibodies were employed to quantify the isozyme concentration in rat liver during development. 2. Total enzyme activity decreases towards birth and reaches a minimum on the 3rd postnatal day, but the activity increases dramatically after weaning. 3. Immunoprecipitation revealed that the M2 type predominates in the prenatal period but decreases sharply just before birth. 4. The L isozyme contribution is augmented upon weaning and is sustained until the rat is adult and a L/M ratio of 9:1 is maintained. 5. By means of incorporation studies with [3H]leucine followed by immunoprecipitation, the increase in L-type activity when approaching term and after weaning is explained by a twofold increase in its rate of synthesis coupled with a concomitant reduction of the M2-type synthesis.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PkmRatliver development  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pkm  (pyruvate kinase M1/2)


Additional Information