RGD Reference Report - Intracranial stereotaxic cannulation for development of orthotopic glioblastoma allograft in Sprague-Dawley rats and histoimmunopathological characterization of the brain tumor.

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Intracranial stereotaxic cannulation for development of orthotopic glioblastoma allograft in Sprague-Dawley rats and histoimmunopathological characterization of the brain tumor.
Authors:	Karmakar, S Olive, MF Banik, NL Ray, SK
Citation:	Karmakar S, etal., Neurochem Res. 2007 Dec;32(12):2235-42. Epub 2007 Aug 15.
RGD ID:	2293131
Pubmed:	PMID:17701349 (View Abstract at PubMed)
DOI:	DOI:10.1007/s11064-007-9450-6 (Journal Full-text)
Glioblastoma is the most common brain tumor that causes significant mortality annually. Limitations of the current therapeutic regimens warrant development of new techniques and treatment strategies in orthotopic animal model for better management of this devastating brain cancer. There are only a few experimental orthotopic models of glioblastoma for pre-clinical testing. In the present investigation, we successfully implanted rat C6 cells via intracranial stereotaxic cannulation in adult Sprague-Dawley rats for development and histoimmunopathological characterization of an advanced orthotopic glioblastoma allograft model, which could be useful for investigating the course of glioblastoma development as well as for testing efficacy of new therapeutic agents. The orthotopic glioblastoma allograft was generated by intracerebral injection of rat C6 cells through a guide-cannula system and after 21 post-inoculation days the brain tumor was characterized by histoimmunopathological experiments. Histological staining and immunofluorescent labelings for TERT, VEGF, Bcl-2, survivin, XIAP, and GFAP revealed the distinct characteristics of glioblastoma in C6 allograft, which could be useful as a target for treatment with emerging new therapeutic agents. Our investigation indicated the successful development of intracranial cannulated orthotopic glioblastoma allograft in adult Sprague-Dawley rats, making it as a useful animal model of glioblastoma for pre-clinical evaluation of various therapeutic strategies for the management of glioblastoma.

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Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
BIRC5	Human	high grade glioma		ISO	Birc5 (Rattus norvegicus)	protein:increased expression:brain	RGD
Birc5	Rat	high grade glioma		IEP		protein:increased expression:brain	RGD
Birc5	Mouse	high grade glioma		ISO	Birc5 (Rattus norvegicus)	protein:increased expression:brain	RGD

Objects Annotated

Genes (Rattus norvegicus)

Birc5 (baculoviral IAP repeat-containing 5)

Genes (Mus musculus)

Birc5 (baculoviral IAP repeat-containing 5)

Genes (Homo sapiens)

BIRC5 (baculoviral IAP repeat containing 5)

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Intracranial stereotaxic cannulation for development of orthotopic glioblastoma allograft in Sprague-Dawley rats and histoimmunopathological characterization of the brain tumor.