RGD Reference Report - Characterization of rat OX40 ligand by monoclonal antibody. - Rat Genome Database

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Characterization of rat OX40 ligand by monoclonal antibody.

Authors: Satake, Y  Akiba, H  Takeda, K  Atsuta, M  Yagita, H  Okumura, K 
Citation: Satake Y, etal., Biochem Biophys Res Commun. 2000 Apr 21;270(3):1041-8.
RGD ID: 2289071
Pubmed: PMID:10772947   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.2000.2560   (Journal Full-text)

OX40 (CD134) is a member of the tumor necrosis factor (TNF) receptor superfamily first identified as a rat T cell activation marker. We previously identified the rat ligand for OX40 (OX40L) by molecular cloning. In the present study, we newly generated an anti-rat OX40L mAb (ATM-2) that can inhibit the binding of OX40 to rat OX40L and thus efficiently inhibits the T cell costimulatory activity of rat OX40L. Flow cytometric analyses using ATM-2 and an anti-rat OX40 mAb (MRC OX40) indicated that OX40 was inducible on splenic CD4(+) T cells by stimulation with immobilized anti-CD3 mAb, while OX40L was not expressed on resting or activated T cells. OX40L was expressed on splenic B cells after stimulation with lipopolysaccharide (LPS), but not on peritoneal macrophages. Interestingly, splenic dendritic cells (DC) expressed OX40L constitutively, which was further upregulated by LPS stimulation. The potent costimulatory activities of splenic DC for anti-CD3-stimulated rat CD4(+) T cell proliferation and cytokine (IL-2, IFN-gamma, IL-10, and IL-13) production were substantially inhibited by ATM-2. These results indicated that OX40L is expressed on professional antigen-presenting cells (APC), and may be involved in humoral immune responses via T-B interaction and in cellular immune responses via T-DC interaction in the rat system.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Tnfsf4Ratcytokine production  IMP  RGD 
Tnfsf4RatT cell proliferation  IMP  RGD 

Molecular Function
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Tnfsf4Rattumor necrosis factor receptor superfamily binding  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Tnfsf4  (TNF superfamily member 4)


Additional Information