RGD Reference Report - Inhibited HDAC3 or Elevated MicroRNA-494-3p Plays a Protective Role in Myocardial Ischemia-Reperfusion Injury via Suppression of BRD4. - Rat Genome Database

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Inhibited HDAC3 or Elevated MicroRNA-494-3p Plays a Protective Role in Myocardial Ischemia-Reperfusion Injury via Suppression of BRD4.

Authors: Zheng, Wuyang  Xie, Qiang  Zhang, Ziguan  Li, Jun  Fang, Lihuan  Li, Weihua 
Citation: Zheng W, etal., Mol Neurobiol. 2021 Sep;58(9):4268-4279. doi: 10.1007/s12035-021-02369-y. Epub 2021 May 12.
RGD ID: 155883171
Pubmed: PMID:33982231   (View Abstract at PubMed)
DOI: DOI:10.1007/s12035-021-02369-y   (Journal Full-text)

Increased histone deacetylase 3 (HDAC3) has been demonstrated to contribute to the pathogenesis of myocardial ischemia-reperfusion injury (MI/RI). Therefore, the goal of this study was to investigate how HDAC3 regulated MI/RI by mediating microRNA (miR)-494-3p/dromodomain-containing protein 4 (BRD4) axis. The MI/RI model was established by ligating the right anterior descending coronary artery. Cardiomyocytes from newborn mice were treated with hypoxia/reoxygenation (H/R). Gain-of-function and loss-of-function approaches were implemented to figure out the roles of miR-494-3p and HDAC3 in MI/RI. miR-494-3p, HDAC3, and BRD4 in myocardial tissues of mice with MI/RI and H/R-treated cardiomyocytes were detected. The relationships between miR-494-3p and HDAC3 and BRD4 were identified. Reduced miR-494-3p and upregulated HDAC3 and BRD4 exhibited in myocardial tissues of mice with MI/RI and H/R-treated cardiomyocytes. Inhibited HDAC3 or elevated miR-494-3p repressed the inflammation and apoptosis, improved cardiac function, and ameliorated myocardial injury in myocardial tissues of mice with MI/RI. Suppression of HDAC3 or elevation of miR-494-3p depressed inflammation and apoptosis and promoted cell viability of primary cardiomyocytes. miR-494-3p targeted BRD4. The study concludes that suppressed HDAC3 plays a protective role in MI/RI by upregulation of miR-494-3p and inhibition of BRD4, which could be helpful for MI/RI therapy.



RGD Manual Disease Annotations    Click to see Annotation Detail View
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
BRD4HumanMyocardial Reperfusion Injury  ISOBrd4 (Mus musculus)mRNA and protein:increased expression:heartRGD 
Brd4RatMyocardial Reperfusion Injury  ISOBrd4 (Mus musculus)mRNA and protein:increased expression:heartRGD 
Brd4MouseMyocardial Reperfusion Injury  IEP mRNA and protein:increased expression:heartRGD 
HDAC3HumanMyocardial Reperfusion Injury amelioratesISOHdac3 (Mus musculus) RGD 
Hdac3RatMyocardial Reperfusion Injury amelioratesISOHdac3 (Mus musculus) RGD 
Hdac3MouseMyocardial Reperfusion Injury amelioratesIMP  RGD 
MIR494HumanMyocardial Reperfusion Injury amelioratesISOMir494 (Mus musculus) RGD 
Mir494MouseMyocardial Reperfusion Injury amelioratesIDA  RGD 
Mir494RatMyocardial Reperfusion Injury amelioratesISOMir494 (Mus musculus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Brd4  (bromodomain containing 4)
Hdac3  (histone deacetylase 3)
Mir494  (microRNA 494)

Genes (Mus musculus)
Brd4  (bromodomain containing 4)
Hdac3  (histone deacetylase 3)
Mir494  (microRNA 494)

Genes (Homo sapiens)
BRD4  (bromodomain containing 4)
HDAC3  (histone deacetylase 3)
MIR494  (microRNA 494)


Additional Information