RGD Reference Report - Silencing interleukin 1α underlies a novel inhibitory role of miR-181c-5p in alleviating low-grade inflammation of rats with irritable bowel syndrome. - Rat Genome Database

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Silencing interleukin 1α underlies a novel inhibitory role of miR-181c-5p in alleviating low-grade inflammation of rats with irritable bowel syndrome.

Authors: Ji, Li-Jiang  Li, Fang  Zhao, Ping  Weng, Li-Ping  Wei, Jun  Yan, Jing  Liu, Li-Na 
Citation: Ji LJ, etal., J Cell Biochem. 2019 Sep;120(9):15268-15279. doi: 10.1002/jcb.28794. Epub 2019 Jun 6.
RGD ID: 15023467
Pubmed: PMID:31172560   (View Abstract at PubMed)
DOI: DOI:10.1002/jcb.28794   (Journal Full-text)

Irritable bowel syndrome (IBS) is a common disorder of unknown etiology. Studies have found a close relation between IBS and microRNAs (miRNAs), but the study concerning the relationship between IBS and miR-181c-5p in IBS is still blank. Thus, this study aims to explore the role of miR-181c-5p in IBS via interleukin 1α (IL1A). Initially, microarray analysis was used to retrieve the genes related to IBS and to predict miRNAs regulating IL1A gene. IBS model was then established with abdominal withdraw reflection (AWR) and Bristol stool grading in mice measured. Afterwards, the functional role of miR-181c-5p in IBS was determined using the ectopic expression, depletion and reporter assay experiments, as well as miR-181c-5p and IL1A expression detected. Subsequently, expression of tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2), and IL-6 were detected to further determine the effects of miR-181c-5p and IL1A on inflammation in IBS. miR-181c-5p and IL1A might be involved in IBS. miR-181c-5p was found to be decreased while IL1A was increased in IBS rats. In addition, miR-181c-5p could target and inhibit expression of IL1A, and IBS mice exhibited elevated AWR and Bristol stool grading, namely 6 to 7 points (70.4 [38 of 54]). Moreover, with the overexpression of miR-181c-5p or silencing of IL1A, the expression of TNF-α, IL-2, and IL-6 was decreased. Collectively, this study suggested that overexpressed miR-181c-5p could silence IL1A, thus inhibiting low-grade inflammation in IBS rats. miR-181c-5p/IL1A is expected to serve as a novel target for the treatment of IBS.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Il1aRatpositive regulation of interleukin-2 production acts_upstream_ofIMP PMID:31172560ARUK-UCL 
Il1aRatpositive regulation of interleukin-6 production acts_upstream_ofIMP PMID:31172560ARUK-UCL 
Il1aRatpositive regulation of tumor necrosis factor production acts_upstream_ofIMP PMID:31172560ARUK-UCL 

Objects Annotated

Genes (Rattus norvegicus)
Il1a  (interleukin 1 alpha)


Additional Information