RGD Reference Report - Differential Neuroproteomic and Systems Biology Analysis of Spinal Cord Injury. - Rat Genome Database

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Differential Neuroproteomic and Systems Biology Analysis of Spinal Cord Injury.

Authors: Moghieb, Ahmed  Bramlett, Helen M  Das, Jyotirmoy H  Yang, Zhihui  Selig, Tyler  Yost, Richard A  Wang, Michael S  Dietrich, W Dalton  Wang, Kevin K W 
Citation: Moghieb A, etal., Mol Cell Proteomics. 2016 Jul;15(7):2379-95. doi: 10.1074/mcp.M116.058115. Epub 2016 May 5.
RGD ID: 13506243
Pubmed: PMID:27150525   (View Abstract at PubMed)
PMCID: PMC4937511   (View Article at PubMed Central)
DOI: DOI:10.1074/mcp.M116.058115   (Journal Full-text)

Acute spinal cord injury (SCI) is a devastating condition with many consequences and no known effective treatment. Although it is quite easy to diagnose traumatic SCI, the assessment of injury severity and projection of disease progression or recovery are often challenging, as no consensus biomarkers have been clearly identified. Here rats were subjected to experimental moderate or severe thoracic SCI. At 24h and 7d postinjury, spinal cord segment caudal to injury center versus sham samples was harvested and subjected to differential proteomic analysis. Cationic/anionic-exchange chromatography, followed by 1D polyacrylamide gel electrophoresis, was used to reduce protein complexity. A reverse phase liquid chromatography-tandem mass spectrometry proteomic platform was then utilized to identify proteome changes associated with SCI. Twenty-two and 22 proteins were up-regulated at 24 h and 7 day after SCI, respectively; whereas 19 and 16 proteins are down-regulated at 24 h and 7 day after SCI, respectively, when compared with sham control. A subset of 12 proteins were identified as candidate SCI biomarkers - TF (Transferrin), FASN (Fatty acid synthase), NME1 (Nucleoside diphosphate kinase 1), STMN1 (Stathmin 1), EEF2 (Eukaryotic translation elongation factor 2), CTSD (Cathepsin D), ANXA1 (Annexin A1), ANXA2 (Annexin A2), PGM1 (Phosphoglucomutase 1), PEA15 (Phosphoprotein enriched in astrocytes 15), GOT2 (Glutamic-oxaloacetic transaminase 2), and TPI-1 (Triosephosphate isomerase 1), data are available via ProteomeXchange with identifier PXD003473. In addition, Transferrin, Cathepsin D, and TPI-1 and PEA15 were further verified in rat spinal cord tissue and/or CSF samples after SCI and in human CSF samples from moderate/severe SCI patients. Lastly, a systems biology approach was utilized to determine the critical biochemical pathways and interactome in the pathogenesis of SCI. Thus, SCI candidate biomarkers identified can be used to correlate with disease progression or to identify potential SCI therapeutic targets.



RGD Manual Disease Annotations    Click to see Annotation Detail View
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
GOT2HumanSpinal Cord Injuries  ISOGot2 (Rattus norvegicus)protein:increased expression:spinal cord (rat)RGD 
Got2RatSpinal Cord Injuries  IEP protein:increased expression:spinal cord (rat)RGD 
Got2MouseSpinal Cord Injuries  ISOGot2 (Rattus norvegicus)protein:increased expression:spinal cord (rat)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Got2  (glutamic-oxaloacetic transaminase 2)

Genes (Mus musculus)
Got2  (glutamatic-oxaloacetic transaminase 2, mitochondrial)

Genes (Homo sapiens)
GOT2  (glutamic-oxaloacetic transaminase 2)


Additional Information