RGD Reference Report - Expression of rat kidney anion exchanger 1 in type A intercalated cells in metabolic acidosis and alkalosis. - Rat Genome Database

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Expression of rat kidney anion exchanger 1 in type A intercalated cells in metabolic acidosis and alkalosis.

Authors: Huber, S  Asan, E  Jöns, T  Kerscher, C  Püschel, B  Drenckhahn, D 
Citation: Huber S, etal., Am J Physiol. 1999 Dec;277(6 Pt 2):F841-9.
RGD ID: 13208945
Pubmed: PMID:10600930   (View Abstract at PubMed)

By enzyme-linked in situ hybridization (ISH), direct evidence is provided that acid-secreting intercalated cells (type A IC) of both the cortical and medullary collecting ducts of the rat kidney selectively express the mRNA of the kidney splice variant of anion exchanger 1 (kAE1) and no detectable levels of the erythrocyte AE1 (eAE1) mRNA. Using single-cell quantification by microphotometry of ISH enzyme reaction, medullary type A IC were found to contain twofold higher kAE1 mRNA levels compared with cortical type A IC. These differences correspond to the higher intensity of immunostaining in medullary versus cortical type A IC. Chronic changes of acid-base status induced by addition of NH(4)Cl (acidosis) or NaHCO3 (alkalosis) to the drinking water resulted in up to 35% changes of kAE1 mRNA levels in both cortical and medullary type A IC. These experiments provide direct evidence at the cellular level of kAE1 expression in type A IC and show moderate capacity of type A IC to respond to changes of acid-base status by modulation of kAE1 mRNA levels.



RGD Manual Disease Annotations    Click to see Annotation Detail View
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SLC4A1HumanAlkalosis  ISOSlc4a1 (Rattus norvegicus)mRNA and protein:decreased expression:kidney:RGD 
Slc4a1RatAlkalosis  IEP mRNA and protein:decreased expression:kidney:RGD 
Slc4a1MouseAlkalosis  ISOSlc4a1 (Rattus norvegicus)mRNA and protein:decreased expression:kidney:RGD 
SLC4A1Humanrenal tubular acidosis  ISOSlc4a1 (Rattus norvegicus)mRNA and protein:increased expression:kidney:RGD 
Slc4a1Ratrenal tubular acidosis  IEP mRNA and protein:increased expression:kidney:RGD 
Slc4a1Mouserenal tubular acidosis  ISOSlc4a1 (Rattus norvegicus)mRNA and protein:increased expression:kidney:RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc4a1Ratresponse to acidic pH  IEP  RGD 
Slc4a1Ratresponse to alkaline pH  IEP  RGD 

Cellular Component
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc4a1Ratbasolateral plasma membrane  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc4a1  (solute carrier family 4 member 1 (Diego blood group))

Genes (Mus musculus)
Slc4a1  (solute carrier family 4 (anion exchanger), member 1)

Genes (Homo sapiens)
SLC4A1  (solute carrier family 4 member 1 (Diego blood group))


Additional Information