RGD Reference Report - Microphthalmia-associated transcription factor and tyrosinase as markers of melanoma cells in blood of patients with melanoma. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models   new Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources

Microphthalmia-associated transcription factor and tyrosinase as markers of melanoma cells in blood of patients with melanoma.

Authors: Samija, Ivan  Lukac, Josip  Maric-Brozic, Jasmina  Kusic, Zvonko 
Citation: Samija I, etal., Croat Med J. 2004 Apr;45(2):142-8.
RGD ID: 13204752
Pubmed: PMID:15103749   (View Abstract at PubMed)


AIM: To investigate whether analysis of microphthalmia-associated transcription factor (MITF) as an additional marker to tyrosinase in melanoma patients can improve the detection of circulating melanoma cells by reverse-transcription-polymerase chain reaction (RT-PCR).
METHODS: Blood samples were taken from 33 patients with metastatic melanoma. RNA was isolated from mononuclear cell fraction of the blood and reversely transcribed into the complementary DNA (cDNA). The cDNA was assayed by PCR for the expression of tyrosinase and MITF. Peripheral blood samples from 15 healthy subjects were used as controls.
RESULTS: The threshold for detection of both tyrosinase and MITF was set low enough to detect 50 melanoma cells in 10 mL of healthy volunteer blood in the relative ratio of one melanoma cell in 0.82 x 106 peripheral blood leukocytes. Out of 33 blood samples from metastatic melanoma patients, 5 were positive for both tyrosinase and MITF, 7 for tyrosinase only, and 5 for MITF only. All samples from healthy volunteers were negative for both tyrosinase and MITF.
CONCLUSION: Determination of MITF marker in addition to tyrosinase improved the detection of circulating melanoma cells in patients with metastatic melanoma.



RGD Manual Disease Annotations    Click to see Annotation Detail View
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MITFHumanmelanoma  IEP mRNA:increased expression:bloodRGD 
MitfRatmelanoma  ISOMITF (Homo sapiens)mRNA:increased expression:bloodRGD 
MitfMousemelanoma  ISOMITF (Homo sapiens)mRNA:increased expression:bloodRGD 

Objects Annotated

Genes (Rattus norvegicus)
Mitf  (melanocyte inducing transcription factor)

Genes (Mus musculus)
Mitf  (melanogenesis associated transcription factor)

Genes (Homo sapiens)
MITF  (melanocyte inducing transcription factor)


Additional Information