RGD Reference Report - A G protein-coupled receptor kinase induces Xenopus oocyte maturation. - Rat Genome Database

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A G protein-coupled receptor kinase induces Xenopus oocyte maturation.

Authors: Wang, J  Liu, XJ 
Citation: Wang J and Liu XJ, J Biol Chem 2003 May 2;278(18):15809-14. Epub 2003 Feb 24.
RGD ID: 1304363
Pubmed: PMID:12600992   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M300320200   (Journal Full-text)

Several recent studies have suggested that resumption of oocyte meiosis, indicated by germinal vesicle breakdown or GVBD, involves inhibition of endogenous heterotrimeric G proteins in both frogs and mice. These studies imply that a heterotrimeric G protein(s), and hence its upstream activator (a G protein-coupled receptor or GpCR), is activated in prophase oocytes and is responsible for maintaining meiosis arrest. To test the existence and function of this putative GpCR, we utilized a mammalian G-protein-coupled receptor kinase (GRK3) and beta-arrestin-2, which together are known to cause GpCR desensitization. Injection of mRNA for rat GRK3 caused hormone-independent GVBD. The kinase activity of GRK3 was essential for GVBD induction as its kinase-dead mutant (GRK3-K220R) was completely ineffective. Another GRK3 mutant (GRK3-DeltaC), which lacked the C-terminal G(betagamma)-binding domain and which was not associated with oocyte membranes, also failed to induce GVBD. Furthermore, injection of rat beta-arrestin-2 mRNA also induced hormone-independent GVBD. Several inhibitors of clathrin-mediated receptor endocytosis (the clathrin-binding domain of beta-arrestin-2, concanavalin A, and monodansyl cadaverine) significantly reduced the abilities of GRK3/beta-arrestin-2 to induce GVBD. These results support the central role of a yet-unidentified GpCR in maintaining prophase arrest in frog oocytes and provide a potential means for its molecular identification.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Arrb2Ratendocytosis  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Arrb2  (arrestin, beta 2)


Additional Information