RGD Reference Report - Regulation of a bifunctional mRNA results in synthesis of secreted and nuclear probasin. - Rat Genome Database

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Regulation of a bifunctional mRNA results in synthesis of secreted and nuclear probasin.

Authors: Spence, AM  Sheppard, PC  Davie, JR  Matuo, Y  Nishi, N  McKeehan, WL  Dodd, JG  Matusik, RJ 
Citation: Spence AM, etal., Proc Natl Acad Sci U S A 1989 Oct;86(20):7843-7.
RGD ID: 1299536
Pubmed: PMID:2682630   (View Abstract at PubMed)
PMCID: PMC298167   (View Article at PubMed Central)

Probasin, a rat prostatic protein, is statistically related to members of a protein family that includes serum, cellular, and nuclear proteins. In vivo, probasin appears both in the secretions and in the nucleus of prostatic epithelial cells. Using primer extension and S1 nuclease protection assays we detected only one probasin mRNA. Thus, the localization of this protein to two separate cellular regions must be encoded by this one mRNA. Furthermore, in vitro translation of synthetic probasin mRNA demonstrated that a protein containing a signal peptide and a protein lacking a signal peptide were synthesized by initiation at different AUG codons. These data are consistent with a mechanism of translational regulation of a eukaryotic bifunctional mRNA.

Objects referenced in this article
Gene Pbsn probasin Rattus norvegicus

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