RGD Reference Report - A model of stereocilia adaptation based on single molecule mechanical studies of myosin I. - Rat Genome Database

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A model of stereocilia adaptation based on single molecule mechanical studies of myosin I.

Authors: Batters, C  Wallace, MI  Coluccio, LM  Molloy, JE 
Citation: Batters C, etal., Philos Trans R Soc Lond B Biol Sci. 2004 Dec 29;359(1452):1895-905.
RGD ID: 11532774
Pubmed: PMID:15647165   (View Abstract at PubMed)
PMCID: PMC1693475   (View Article at PubMed Central)
DOI: DOI:10.1098/rstb.2004.1559   (Journal Full-text)

We have used an optical tweezers-based apparatus to perform single molecule mechanical experiments using the unconventional myosins, Myo1b and Myo1c. The single-headed nature and slow ATPase kinetics of these myosins make them ideal for detailed studies of the molecular mechanism of force generation by acto-myosin. Myo1c exhibits several features that have not been seen using fast skeletal muscle myosin II. (i) The working stroke occurs in two, distinct phases, producing an initial 3 nm and then a further 1.5 nm of movement. (ii) Two types of binding interaction were observed: short-lived ATP-independent binding events that produced no movement and longer-lived, ATP-dependent events that produced a full working stroke. The stiffness of both types of interaction was similar. (iii) In a new type of experiment, using feedback to apply controlled displacements to a single acto-myosin cross-bridge, we found abrupt changes in force during attachment of the acto-Myo1b cross-bridge, a result that is consistent with the classical 'T2' behaviour of single muscle fibres. Given that these myosins might exhibit the classical T2 behaviour, we propose a new model to explain the slow phase of sensory adaptation of the hair cells of the inner ear.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Myo1bRatactin filament binding  IDA  RGD 
Myo1cRatactin filament binding  IDA  RGD 
Myo1bRatmicrofilament motor activity  IDA  RGD 
Myo1cRatmicrofilament motor activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Myo1b  (myosin Ib)
Myo1c  (myosin 1C)


Additional Information