RGD Reference Report - Calcineurin Agamma is a Functional Phosphatase That Modulates Synaptic Vesicle Endocytosis. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models   new Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources

Calcineurin Agamma is a Functional Phosphatase That Modulates Synaptic Vesicle Endocytosis.

Authors: Cottrell, JR  Li, B  Kyung, JW  Ashford, CJ  Mann, JJ  Horvath, TL  Ryan, TA  Kim, SH  Gerber, DJ 
Citation: Cottrell JR, etal., J Biol Chem. 2016 Jan 22;291(4):1948-56. doi: 10.1074/jbc.M115.705319. Epub 2015 Dec 1.
RGD ID: 11532172
Pubmed: PMID:26627835   (View Abstract at PubMed)
PMCID: PMC4722470   (View Article at PubMed Central)
DOI: DOI:10.1074/jbc.M115.705319   (Journal Full-text)

Variation in PPP3CC, the gene that encodes the gamma isoform of the calcineurin catalytic subunit, has been reported to be associated with schizophrenia. Because of its low expression level in most tissues, there has been little research devoted to the specific function of the calcineurin Agamma (CNAgamma) versus the calcineurin Aalpha (CNAalpha) and calcineurin Abeta (CNAbeta) catalytic isoforms. Consequently, we have a limited understanding of the role of altered CNAgamma function in psychiatric disease. In this study, we demonstrate that CNAgamma is present in the rodent and human brain and dephosphorylates a presynaptic substrate of calcineurin. Through a combination of immunocytochemistry and immuno-EM, we further show that CNAgamma is localized to presynaptic terminals in hippocampal neurons. Critically, we demonstrate that RNAi-mediated knockdown of CNAgamma leads to a disruption of synaptic vesicle cycling in cultured rat hippocampal neurons. These data indicate that CNAgamma regulates a critical aspect of synaptic vesicle cycling and suggest that variation in PPP3CC may contribute to psychiatric disease by altering presynaptic function.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ppp3ccRatpositive regulation of synaptic vesicle endocytosis  IMP  RGD 
Ppp3ccRatregulation of synaptic vesicle endocytosis involved_inIDA PMID:26627835SynGO 
Ppp3ccRatregulation of synaptic vesicle endocytosis involved_inIMP PMID:26627835SynGO 

Cellular Component
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ppp3ccRatglutamatergic synapse is_active_inIDA PMID:26627835SynGO 
Ppp3ccRatglutamatergic synapse is_active_inIMP PMID:26627835SynGO 
Ppp3ccRatpresynapse  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ppp3cc  (protein phosphatase 3 catalytic subunit gamma)

Objects referenced in this article
Gene Ppp3cb protein phosphatase 3 catalytic subunit beta Rattus norvegicus

Additional Information