RGD Reference Report - MiR-31 Functions as a Tumor Suppressor in Lung Adenocarcinoma Mainly by Targeting HuR. - Rat Genome Database

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MiR-31 Functions as a Tumor Suppressor in Lung Adenocarcinoma Mainly by Targeting HuR.

Authors: Xu, H  Ma, J  Zheng, J  Wu, J  Qu, C  Sun, F  Xu, S 
Citation: Xu H, etal., Clin Lab. 2016;62(4):711-8.
RGD ID: 11250998
Pubmed: PMID:27215092   (View Abstract at PubMed)

BACKGROUND: Gene expression is widely regulated by miRNAs and RNA binding proteins. In this study, we mainly focused on miR-31 and a RNA binding protein, HuR (Hu antigen R). METHODS: The levels of miR-31 and HuR in lung carcinoma cells and lung cancer tissues were explored using RT-qPCR and western blot, respectively. Luciferase reporter assay was used to determine the target gene of miR-31. Cell apoptosis and migration were studied using flow cytometry and the transwell invasion assay. The down-stream genes of HuR were explored with western blot assay. RESULTS: miR-31 was decreased in lung carcinoma cells and lung cancer tissues, while the protein level of HuR was increased. HuR was the target gene of miR-31. Inhibition of miR-31 and overexpression of HuR resulted in the upregulation of cyclins A2, B1, D1 and VEGF (vascular endothelial growth factor). Furthermore, overexpression of miR-31 prompted lung cancer cell apoptosis and inhibited cell migration. CONCLUSIONS: Reduction of miR-31 expression enhanced lung cancer proliferation and migration by repressing HuR expression.



RGD Manual Disease Annotations    Click to see Annotation Detail View
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MIR31Humanlung adenocarcinoma  IEP RNA:increased expression:lung (human)RGD 
Mir31Mouselung adenocarcinoma  ISOMIR31 (Homo sapiens)RNA:increased expression:lung (human)RGD 
Mir31Ratlung adenocarcinoma  ISOMIR31 (Homo sapiens)RNA:increased expression:lung (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mir31  (microRNA 31)

Genes (Mus musculus)
Mir31  (microRNA 31)

Genes (Homo sapiens)
MIR31  (microRNA 31)


Additional Information