RGD Reference Report - Induction of ER stress in response to oxygen-glucose deprivation of cortical cultures involves the activation of the PERK and IRE-1 pathways and of caspase-12. - Rat Genome Database

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Induction of ER stress in response to oxygen-glucose deprivation of cortical cultures involves the activation of the PERK and IRE-1 pathways and of caspase-12.

Authors: Badiola, N  Penas, C  Minano-Molina, A  Barneda-Zahonero, B  Fado, R  Sanchez-Opazo, G  Comella, JX  Sabria, J  Zhu, C  Blomgren, K  Casas, C  Rodriguez-Alvarez, J 
Citation: Badiola N, etal., Cell Death Dis. 2011 Apr 28;2:e149. doi: 10.1038/cddis.2011.31.
RGD ID: 10054236
Pubmed: PMID:21525936   (View Abstract at PubMed)
PMCID: PMC3122062   (View Article at PubMed Central)
DOI: DOI:10.1038/cddis.2011.31   (Journal Full-text)

Disturbance of calcium homeostasis and accumulation of misfolded proteins in the endoplasmic reticulum (ER) are considered contributory components of cell death after ischemia. However, the signal-transducing events that are activated by ER stress after cerebral ischemia are incompletely understood. In this study, we show that caspase-12 and the PERK and IRE pathways are activated following oxygen-glucose deprivation (OGD) of mixed cortical cultures or neonatal hypoxia-ischemia (HI). Activation of PERK led to a transient phosphorylation of eIF2alpha, an increase in ATF4 levels and the induction of gadd34 (a subunit of an eIF2alpha-directed phosphatase). Interestingly, the upregulation of ATF4 did not lead to an increase in the levels of CHOP. Additionally, IRE1 activation was mediated by the increase in the processed form of xbp1, which would be responsible for the observed expression of edem2 and the increased levels of the chaperones GRP78 and GRP94. We were also able to detect caspase-12 proteolysis after HI or OGD. Processing of procaspase-12 was mediated by NMDA receptor and calpain activation. Moreover, our data suggest that caspase-12 activation is independent of the unfolded protein response activated by ER stress.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ppp1r15aRatcellular response to hypoxia  IEP oxygen-glucose deprivationRGD 

Objects Annotated

Genes (Rattus norvegicus)
Ppp1r15a  (protein phosphatase 1, regulatory subunit 15A)


Additional Information