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Strain Registration

Strain: W-Tg(tetO-Pou5f1,-Klf4,-Sox2,Ubc-rtTA,-EGFP)T1-3Hina

Symbol: W-Tg(tetO-Pou5f1,-Klf4,-Sox2,Ubc-rtTA,-EGFP)T1-3Hina
Strain: W-Tg(tetO-Pou5f1,-Klf4,-Sox2,Ubc-rtTA,-EGFP)T1-3
Substrain: Hina
RGD ID: 9685755
Citation ID: RRID:RGD_9685755
Ontology ID: RS:0003856
Also Known As: Dox-dependent re-programmable rat; W-Tg(TRE-mOKS Ubc-rTet I/EGFP)T1-3/Hina; NBRP Rat No: 0695
Type: transgenic
Available Source: National BioResource Project for the Rat in Japan
Origination: National BioResource Project for the Rat in Japan
Description: Doxycycline induced mouse Oct3/4, Klf4, Sox2/ Ubc-promoter EGFP was introduced into embryonic fibroblast of Wistar rat (Slc:Wistar)by replication incompetent type lentivirus vector and iPS cells were induced. Chimeric rats (male) were generated from this iPS cells, and crossed with the wild-type Wistar rat (female).
Coat Color: Albino
Last Known Status: Cryopreserved Embryo
Research Usage Development






References

References - curated
# Reference Title Reference Citation
1. Strains registered by the National Bio Resource Project for the Rat in Japan Personal Communication with NBRP
2. RGD Strain RSO annotation pipeline RGD Automated Pipelines

Region


Additional Information

RGD Curation Notes
Note Type Note Reference
strain_characteristics The reprogramming can be induced by re-induction of transgene (Oct3/4, Klf4, Sox2) in the presence of Doxycycline. iPS cells can be induced from somatic cells under the optimal culture condition. 1302502
strain_reproduction Maintained in heterozygous condition, Good breeding performance. About a half of rats has EGFP expression. 1302502

Nomenclature History
Date Current Symbol Current Name Previous Symbol Previous Name Description Reference Status
2015-05-27 W-Tg(tetO-Pou5f1,-Klf4,-Sox2,Ubc-rtTA,-EGFP)T1-3Hina    W-Tg(tetO-Pou5f1,-Klf4,-Sox2,Ubc-rtTA,-EGFP)T1-3Hina    Name updated 68913 APPROVED
2015-05-27 W-Tg(tetO-Pou5f1,-Klf4,-Sox2,Ubc-rtTA,-EGFP)T1-3Hina    W-Tg(tetO-Pou5f1,-Klf4,-Sox2,Ubc-rtTA,-EGFP)T1-3Hina    Name updated 68913 APPROVED