Strain Report - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models   new Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Strain Registration

Strain: SD-Aif1tm(EGFP)Apps/Mmmc

Symbol: SD-Aif1tm(EGFP)Apps/Mmmc
Strain: SD-Aif1tm(EGFP)
Substrain: AppsMmmc
RGD ID: 152999023
Citation ID: RRID:RGD_152999023
Ontology ID: RS:0005151
Alleles: Aif1tm(EGFP)Apps
Also Known As: SD-Aif1^[tm(EGFP)Apps]/Mmmc
Type: mutant
Available Source: Not Available
Origination: Department of Pharmacology, University of Maryland School of Medicine, Baltimore, Maryland 21201
Description: Applied StemCell, Inc (Milpitas, CA) was contracted to generate the Iba1-EGFP knock-in rat model using CRISPR/Cas9 technology in the Sprague Dawley rat strain. The donor construct inserted consisted of the EGFP coding sequence (minus the first ATG), followed by the 22 amino acid sequence of the porcine teschovirus-1 2A (P2A) self-cleaving peptide, and then the first exon of the rat Iba1 gene immediately downstream of the translational start site. Guide RNA with the following sequence: 5'- TACCCTGCAAATCCTTGCTCTGG-3' targeting the Iba1 gene just downstream of the translational start site were used.
Last Known Status: Unknown
Position
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.2203,646,784 - 3,652,670RGD_MAPPER_PIPELINEmRatBN7.2
Rnor_6.0205,161,350 - 5,167,176RGD_MAPPER_PIPELINERnor6.0
Rnor_5.0207,234,609 - 7,240,454RGD_MAPPER_PIPELINERnor5.0
RGSC_v3.4203,714,428 - 3,716,327RGD_MAPPER_PIPELINERGSC3.4






References

References - curated
# Reference Title Reference Citation
1. Generation of an Iba1-EGFP Transgenic Rat for the Study of Microglia in an Outbred Rodent Strain. VanRyzin JW, etal., eNeuro. 2021 Sep 7;8(5). pii: ENEURO.0026-21.2021. doi: 10.1523/ENEURO.0026-21.2021. Print 2021 Sep-Oct.

Region


Additional Information