Organization: Australian Phenomics Facility, Australian National University
The CRISPR/Cas9 system was used to target exon 11 to create a deletion at codon F508 which was injected into Sprague-Dawley one-cell
embryos (C076 line). One rat
had an allele that contained the desired homology-directed
repair edited TTT deletion and was designated the
Phe508del founder (c.1522_1524delTTT).