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Strain Registration

Strain: FHH-Chr 1BN-Add3em2Mcwi /Roman

Symbol: FHH-Chr 1BN-Add3em2Mcwi /Roman
Strain: FHH-Chr 1BN-Add3em2Mcwi
Substrain: Roman
RGD ID: 150429618
Citation ID: RRID:RGD_150429618
Ontology ID: RS:0005054
Alleles: Add3em2Mcwi
Previously known as: FHH.1BN.Add3 KO
Type: mutant
Source: Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi
Origin: ZFN system was used to Knock out the rat Add3 gene of FHH-Chr 1BN/Mcwi rat embryos.The ZFN mRNA was injected into the pronucleus of fertilized FHH-Chr 1BN/Mcwi embryos and transferred to the oviduct of pseudopregnant females. PCR genotyping of tail biopsies confirmed a 68-bp deletion using forward primer 5'-GCCCCCATGAGTCACTACAC-3' and reverse primer 5'-GCTACAGGAAGCATCTCCTGTG-3'. Founders with Add3 deletion were backcrossed to the parental strain to generate heterozygous F1 rats. Heterozygous F1 siblings were then intercrossed to derive a homozygous KO line used
Genetic Status: Homozygous
Last Known Status: Unknown
Position
Rat AssemblyChrPosition (strand)SourceJBrowse
mRatBN7.21252,147,341 - 252,255,126RGD_MAPPER_PIPELINEmRatBN7.2
Rnor_6.01273,854,195 - 273,961,982RGD_MAPPER_PIPELINERnor6.0
Rnor_5.01281,267,424 - 281,375,203RGD_MAPPER_PIPELINERnor5.0
RGSC_v3.41259,347,673 - 259,455,407RGD_MAPPER_PIPELINERGSC3.4





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Mammalian Phenotype

References

References - curated
# Reference Title Reference Citation
1. A Mutation in γ-Adducin Impairs Autoregulation of Renal Blood Flow and Promotes the Development of Kidney Disease. Fan F, etal., J Am Soc Nephrol. 2020 Apr;31(4):687-700. doi: 10.1681/ASN.2019080784. Epub 2020 Feb 6.

Region


Additional Information


Nomenclature History
Date Current Symbol Current Name Previous Symbol Previous Name Description Reference Status
2021-09-09 FHH-Chr 1BN-Add3em2Mcwi /Roman    FHH-Chr 1BN-Add3em2Mcwi-/- /Roman    Symbol and/or name change 68913 APPROVED
2021-09-09 FHH-Chr 1BN-Add3em2Mcwi-/- /Roman    FHH-Chr 1BN-Add3em2Mcwi-/- /Roman  FHH-Chr 1BN-Sorcs1em1Mcwi-/Sorcs1em1Mcwi-  Symbol and/or name change 68913 APPROVED