RGD Reference Report - miR-335 is involved in the rat epididymal development by targeting the mRNA of RASA1. - Rat Genome Database

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miR-335 is involved in the rat epididymal development by targeting the mRNA of RASA1.

Authors: Wang, J  Ruan, K 
Citation: Wang J and Ruan K, Biochem Biophys Res Commun. 2010 Nov 12;402(2):222-7. doi: 10.1016/j.bbrc.2010.10.002. Epub 2010 Oct 8.
RGD ID: 9999420
Pubmed: (View Article at PubMed) PMID:20933506
DOI: Full-text: DOI:10.1016/j.bbrc.2010.10.002

The expression of 350 miRNAs during the rat epididymal development was profiled by a home-made miRNA microarray recently. The results showed that the expression of miR-335 decreased from postnatal day 7 to 49 in the rat epididymis. Few studies on miR-335's roles in the epididymis and its targets have been reported so far. The bioinformatic analysis indicated that one of miR-335's putative targets is RASA1, a known gene related to cell proliferation and anti-apoptosis, which may contribute to the epididymal development. It was found that the overexpression of miR-335 in NIH/3T3 cells caused the suppression of RASA1 expression. The result of luciferase targeting assay confirmed that the mRNA of RASA1 was targeted by miR-335. In addition, it was also found that the temporal expression of RASA1 was inverse to that of miR-335 during the epididymal development in the rat. These results indicated that the reduction of miR-335 at least in part caused the upregulation of RASA1 in the rat epididymis. Therefore, miR-335 can be involved in the epididymal development by affecting the expression of RASA1.

Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Rasa1  (RAS p21 protein activator 1)

Additional Information