RGD Reference Report - Expression of the high-affinity choline transporter CHT1 in epithelia. - Rat Genome Database

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Expression of the high-affinity choline transporter CHT1 in epithelia.

Authors: Pfeil, U  Haberberger, RV  Lips, KS  Eberling, L  Grau, V  Kummer, W 
Citation: Pfeil U, etal., Life Sci. 2003 Mar 28;72(18-19):2087-90.
RGD ID: 9999384
Pubmed: PMID:12628461   (View Abstract at PubMed)

Uptake of choline by the high-affinity choline transporter CHT1 is the rate-limiting step in neuronal acetylcholine (ACh) synthesis. Here, we investigated by RT-PCR, in-situ hybridisation, immunohistochemistry, and Western blotting whether CHT1 is also expressed in cholinergic epithelia. CHT1-mRNA and -protein were detected in keratinocytes of human skin, rat skin and tongue, the human keratinocyte cell line HaCaT, and the ciliated cells of the rat tracheal epithelium. Immunohistochemically, CHT1 was predominantly localized to the epithelial cell membranes, in case of ciliated tracheal cells it was restricted to the apical membrane. This is the first study to demonstrate the expression of CHT1 in non-neuronal cells. The close apposition of CHT1 to reported sites of localization of choline acetyltransferase in these cells is strongly in favour of ACh synthesis being fuelled by choline uptake via CHT1 in these epithelia.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
apical plasma membrane  IDA 9999384 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc5a7  (solute carrier family 5 member 7)


Additional Information