RGD Reference Report - Effect of prediabetes on membrane bicarbonate transporters in testis and epididymis. - Rat Genome Database

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Effect of prediabetes on membrane bicarbonate transporters in testis and epididymis.

Authors: Bernardino, RL  Martins, AD  Socorro, S  Alves, MG  Oliveira, PF 
Citation: Bernardino RL, etal., J Membr Biol. 2013 Dec;246(12):877-83. doi: 10.1007/s00232-013-9601-4. Epub 2013 Oct 9.
RGD ID: 9999379
Pubmed: PMID:24105628   (View Abstract at PubMed)
DOI: DOI:10.1007/s00232-013-9601-4   (Journal Full-text)

The formation of competent spermatozoa is a complex event that depends on the establishment of adequate environments throughout the male reproductive tract. This includes the control of bicarbonate (HCO(3)(-)) concentration, which plays an essential role in the maintenance of extracellular and intracellular pH (pH(i)) values. Diabetes mellitus alters pHi regulation in mammalian cells, mainly by altering the activity of ion transporters, particularly HCO(3)(-)-dependent mechanisms. Yet, little is known about the effects of this pathology and its prodromal stage, prediabetes, on the membrane transport mechanisms of male reproductive tract cells. Herein, we analyzed protein and mRNA levels of the most relevant HCO(3)(-) transporters of the SLC4 family [anion exchanger 2 (AE2), Na(+)-driven Cl(-)/HCO(3)(-) exchanger (NDCBE), electrogenic Na(+)/HCO(3)(-) cotransporter 1 (NBCe1), electroneutral Na(+)/HCO(3)(-) cotransporter 1 (NBCn1)] in the testis and epididymis of a prediabetic animal model. Firstly, we identified the HCO(3)(-) transporters of the SLC4 family, in both testicular and epididymal tissue. Secondly, although no alterations were detected in protein expression, mRNA levels of NBCe1, NBCn1 and NDCBE were significantly increased in the testis of prediabetic rats. On the other hand, in the epididymis, prediabetes caused an increase of AE2 and a decrease of NDCBE protein levels. These alterations may be translated into changes of HCO(3)(-) transepithelial epididymal fluxes in vivo, which may represent a threat for sperm survival. Moreover, these results provide evidence of the molecular mechanism that may be responsible for the significant increase in abnormal sperm morphology already reported in prediabetic rats.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
prediabetes syndrome  ISOSlc4a2 (Rattus norvegicus)9999379; 9999379protein:increased expression:epididymisRGD 
prediabetes syndrome  ISOSlc4a8 (Rattus norvegicus)9999379; 9999379 RGD 
prediabetes syndrome  IEP 9999379protein:increased expression:epididymisRGD 
prediabetes syndrome  IEP 9999379 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc4a2  (solute carrier family 4 member 2)
Slc4a8  (solute carrier family 4 member 8)

Genes (Mus musculus)
Slc4a2  (solute carrier family 4 (anion exchanger), member 2)
Slc4a8  (solute carrier family 4 (anion exchanger), member 8)

Genes (Homo sapiens)
SLC4A2  (solute carrier family 4 member 2)
SLC4A8  (solute carrier family 4 member 8)


Additional Information