RGD Reference Report - Differential cooperative enzymatic activities of protein disulfide isomerase family in protein folding. - Rat Genome Database

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Differential cooperative enzymatic activities of protein disulfide isomerase family in protein folding.

Authors: Satoh, M  Shimada, A  Kashiwai, A  Saga, S  Hosokawa, M 
Citation: Satoh M, etal., Cell Stress Chaperones. 2005 Autumn;10(3):211-20.
RGD ID: 9999184
Pubmed: PMID:16184766   (View Abstract at PubMed)
PMCID: PMC1226019   (View Article at PubMed Central)

Endoplasmic reticulum (ER)p61, ERp72, and protein disulfide isomerase (PDI), which are members of the PDI family protein, are ubiquitously present in mammalian cells and are thought to participate in disulfide bond formation and isomerization. However, why the 3 different members need to be colocalized in the ER remains an enigma. We hypothesized that each PDI family protein might have different modes of enzymatic activity in disulfide bond formation and isomerization. We purified PDI, ERp61, and ERp72 proteins from rat liver microsomes and compared the effects of each protein on the folding of bovine pancreatic trypsin inhibitor (BPTI). ERp61 and ERp72 accelerated the initial steps more efficiently than did PDI. ERp61 and ERp72, however, accelerated the rate-limiting step less efficiently than did PDI. PDI or ERp72 did not impede the folding of BPTI by each other but rather catalyzed the folding reaction cooperatively with each other. These data suggest that differential enzymatic activities of ERp proteins and PDI represent a complementary contribution of these enzymes to protein folding in the ER.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Pdia3Ratpositive regulation of protein folding  IDA  RGD 
Pdia4Ratpositive regulation of protein folding  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pdia3  (protein disulfide isomerase family A, member 3)
Pdia4  (protein disulfide isomerase family A, member 4)


Additional Information