RGD Reference Report - Subtypes of 20S proteasomes from skeletal muscle. - Rat Genome Database

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Subtypes of 20S proteasomes from skeletal muscle.

Authors: Dahlmann, B  Ruppert, T  Kloetzel, PM  Kuehn, L 
Citation: Dahlmann B, etal., Biochimie. 2001 Mar-Apr;83(3-4):295-9.
RGD ID: 9854635
Pubmed: (View Article at PubMed) PMID:11295489

20S proteasomes from tissues and cells are a mixture of several subtypes. From rat skeletal muscle we have tentatively separated six different subtypes of 20S proteasomes purified from rat skeletal muscle by high-resolution anion exchange chromatography. Immunoblot analysis using antibodies to the beta-subunits LMP2, LMP7 and their constitutive counterparts delta and MB1 revealed that two of the three major subtypes (subtypes I and II) are constitutive proteasomes, whereas two of the three minor subtypes belong to the subpopulation of immuno-proteasomes. Subtype III and IV are intermediate-type proteasomes. Enzymological characterisation of the six subtypes revealed clearly different V(max) values for hydrolysis of fluorogenic peptide substrates as well as significantly different activities measured with a 25-mer polypeptide of the murine cytomegalovirus IE pp89 protein as substrate. Our data show that the properties of 20S proteasomes isolated from a given tissue or cells are always the average of the properties of the whole set of proteasome subtypes.


Gene Ontology Annotations    

Cellular Component

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Psmb9  (proteasome 20S subunit beta 9)

Additional Information